Role of thrombin in interleukin-5 expression from basophils

Biochemical and Biophysical Research Communications
Aiko YamaguchiOsamu Taguchi

Abstract

Interleukin-5 (IL-5) plays a key role in the pathogenesis of bronchial asthma. Thrombin is a procoagulant factor that has been also reported to participate in the inflammatory response by stimulating the secretion of cytokines. Interaction of inflammatory cells with airway epithelial cells may also promote the secretion of cytokines. However, the role of thrombin and cell-to-cell interaction in pathogenesis of allergic inflammation is unclear. In this study, we evaluated the role of thrombin and cell-to-cell interaction in the secretion of IL-5 from basophils. The human basophil cell line KU-812 was used in the assays. Thrombin and co-culture with alveolar epithelial cells significantly stimulated the secretion of IL-5 from KU-812 cells as compared to controls. Secretion of IL-5 was synergistically stimulated when KU-812 cells were incubated in the presence of both thrombin and alveolar epithelial cells. Co-culture of KU-812 cells with epithelial cells significantly increased the expression of tissue factor, an activator of coagulation activation, in a cell dose-dependent manner. Secretion of IL-5 from KU-812 basophils co-cultured with epithelial cells was significantly inhibited by LY294002, an inhibitor of phosphatidylinosito...Continue Reading

References

Jun 29, 1999·Lung·E C GabazzaY Adachi
Jan 15, 2003·Journal of Leukocyte Biology·Clair PhillipsColin R A Hewitt
Feb 3, 2004·Current Pharmaceutical Design·M Rosemblatt, María Rosa Bono
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Aug 25, 2004·Trends in Immunology·A Barry KayDouglas S Robinson

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Citations

Jun 30, 2011·American Journal of Rhinology & Allergy·Shino ShimizuTakeshi Shimizu
Aug 24, 2013·Journal of Thrombosis and Haemostasis : JTH·Y MiyakeE C Gabazza

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