Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats

Zhongguo gu shang = China journal of orthopaedics and traumatology
Xue-Zong WangHong-Sheng Zhan

Abstract

To study role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats. Eighteen male SD rats weighted (200±20) g were randomly divided into 2 groups, namely control and model group, and 9 in each group. Knee OA model group was established by using modified Hulth method in model group. Control group was not treated. Synovial tissue and serum was extracted at 4 and 21 d after operation. Expression of CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13 were detected by real-time PCR respectively. NF-κB p65 protein was detected by Western-blot; serum concentrations of haluronic acid (HA), N-propeptide of type III procollagen(PIIINP) was detected by Elisa. Expression of CD14, ADAMTS-4, and NF-κB p65 in model group were higher than that of control group at 4 and 21 days after operation, while expression of TLR4, IL-1β, TNF-α and MMP-13 were higher than that of control group at 21 days after operation(P<0.01). Concentration of PIIINP and HA in model group were higher than that of control group at 4 days after operation, while there was no significant difference at 21 days after operation. NF-κB pathway could mediate occurrence of KOA by early activating and triggeringg synovial increasingly secreting inflammator...Continue Reading

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