Role of toll-like receptor-4 in lung ischemia-reperfusion injury

The Annals of Thoracic Surgery
Heather E MerryMichael S Mulligan

Abstract

Toll-like receptor-4 has been implicated in modulating ischemia-reperfusion injury in cardiac, hepatic, renal, and cerebral models. However, its role in lung ischemia-reperfusion injury is unknown. We hypothesize that toll-like receptor-4 has a key role in initiating the inflammatory cascade in lung ischemia-reperfusion injury. We used toll-like receptor-4 specific short interference RNA to achieve toll-like receptor-4 knockdown in rats prior to undergoing ischemia and reperfusion. Lungs were explanted and studied for protein expression and markers of lung injury. Additional animals were evaluated for cellular uptake of toll-like receptor-4 short interference RNA. Toll-like receptor-4 short interference RNA localized to the alveolar macrophage. In animals pretreated with toll-like receptor-4 short interference RNA, toll-like receptor-4 expression and mitogen-activated protein kinase phosphorylation were suppressed. Markers of lung injury including permeability index, myeloperoxidase content, and bronchoalveolar lavage inflammatory cell counts were all reduced with toll-like receptor-4 knockdown. Toll-like receptor-4 is critical in the development of lung ischemia-reperfusion injury and its activation in the alveolar macrophage ...Continue Reading

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Citations

Feb 29, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Mamoru TakahashiHiroshi Date
Apr 26, 2015·The Journal of Thoracic and Cardiovascular Surgery·Patrick PhelanMichael S Mulligan
Oct 25, 2016·Experimental Cell Research·Orna AvlasEdith Hochhauser
Mar 6, 2016·Current Opinion in Organ Transplantation·Victor E Laubach, Ashish K Sharma
May 1, 2020·Frontiers in Bioengineering and Biotechnology·N Valerio Dorrello, Gordana Vunjak-Novakovic
Jul 29, 2015·World Journal of Gastroenterology : WJG·Jie WangTai Guo
Dec 30, 2020·The Journal of Surgical Research·Yoshiaki OshimaYoshimi Inagaki
Jan 14, 2021·Transplantation Direct·Tara TalaieChristine L Lau
May 25, 2021·Seminars in Respiratory and Critical Care Medicine·Jake G Natalini, Joshua M Diamond
May 23, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Hailey M ShepherdDaniel Kreisel

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