Role of trehalose in growth at high temperature of Salmonella enterica serovar Typhimurium

Journal of Bacteriology
D CánovasL N Csonka

Abstract

Moderate osmolality can stimulate bacterial growth at temperatures near the upper limit for growth. We investigated the mechanism by which high osmolality enhances the thermotolerance of Salmonella enterica serovar Typhimurium, by isolating bacteriophage MudI1734-induced insertion mutations that blocked the growth-stimulatory effect of 0.2 M NaCl at 45 degrees C. One of these mutations proved to be in the seqA gene (a regulator of initiation of DNA synthesis). Because this gene is cotranscribed with pgm (which encodes phosphoglucomutase), it is likely to be polar on the expression of the pgm gene. Pgm catalyzes the conversion of glucose-6-phosphate to glucose-1-phosphate during growth on glucose, and therefore loss of Pgm results in a deficiency in a variety of cellular constituents derived from glucose-1-phosphate, including trehalose. To test the possibility that the growth defect of the seqA::MudI1734 mutant at high temperature in medium of high osmolality is due to the block in trehalose synthesis, we determined the effect of an otsA mutation, which inactivates the first step of the trehalose biosynthetic pathway. The otsA mutation caused a growth defect at 45 degrees C in minimal medium containing 0.2 M NaCl that was simil...Continue Reading

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Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kathleen O'ConnorLaszlo N Csonka
Apr 5, 2003·Applied and Environmental Microbiology·Keith R ShockleyRobert M Kelly
Jul 8, 2005·Applied and Environmental Microbiology·J E PurvisL O Ingram
Feb 8, 2011·Journal of Bacteriology·Tamara Hoffmann, Erhard Bremer
Mar 25, 2008·Journal of Bacteriology·Thusitha S GunasekeraOleg Paliy
Sep 19, 2012·BMC Microbiology·Mercedes Reina-BuenoCarmen Vargas
Sep 11, 2009·International Journal of Molecular Sciences·Vittorio CapozziGiuseppe Spano
Jan 25, 2016·International Journal of Food Microbiology·Catherine M BurgessOdile Tresse
Mar 1, 2007·The Journal of Infection·Abdelwaheb ChattiAhmed Landoulsi
Jan 18, 2015·Applied and Environmental Microbiology·Shinichi OideMasayuki Inui
Nov 24, 2017·Applied Microbiology and Biotechnology·Kamil PiwowarekIwona Ścibisz
Aug 1, 2009·EcoSal Plus·Karlheinz AltendorfJanet M Wood

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