Role of TRIF Small Interference RNA (siRNA) in Chronic Experimental Allergic Encephalomyelitis (EAE)

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Xichun WangLibo Zhao

Abstract

Multiple sclerosis (MS) is an autoimmune disease causing multifocal demyelination and axonal injuries in the central nervous system (CNS). Toll-interleukin-1 receptor (TIR)-domain containing adaptor protein-inducing interferon beta (TRIF) is an important adaptor protein for Toll-like receptors (TLRs) and can modulate the immune response via regulating cytokine secretion. This study investigated the potential function of TRIF in MS mice via small interference RNA (siRNA). Isolated mouse lymphocytes were processed using TRIF siRNA, followed by RT-PCR assay to quantify TRIF expression level. An experimental allergic encephalomyelitis (EAE) model was prepared in C57BL/6 mice immunized with MOG 35-55. TRIF siRNA or controlled siRNA were intravenously applied to evaluate the neurological function of animals. Serum levels of IFN-γ and IL-2 were observed. Specific siRNA effectively decreased the TRIF expression in mouse dendritic cells and this siRNA improved the EAE severity and neurological scores. Further assays showed that both IFN-γ and IL-2 levels in the siRNA treatment group were significantly lower than in controls. The expression of TRIF can be down-regulated by siRNA, thereby alleviating the severity of EAE via its inhibition...Continue Reading

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Citations

Jun 25, 2019·Frontiers in Neurology·Yang JinSong Bai Xu
Nov 25, 2020·Journal of Molecular Neuroscience : MN·Tarapati RanaSimona Bungau

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Methods Mentioned

BETA
electrophoresis
ELISA
gene knockout

Software Mentioned

SPSS
Primer

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