Role of two-component sensory systems of Salmonella enterica serovar Dublin in the pathogenesis of systemic salmonellosis in cattle.

Microbiology
Gillian D PullingerMark P Stevens

Abstract

Salmonella enterica serovar Dublin (S. Dublin) is associated with enteritis, typhoid and abortion in cattle. Infections are acquired by the oral route, and the bacteria transit through varied anatomical and cellular niches to elicit systemic disease. S. Dublin must therefore sense and respond to diverse extrinsic stimuli to control gene expression in a spatial and temporal manner. Two-component systems (TCSs) play key roles in such processes, and typically contain a membrane-associated sensor kinase (SK) that modifies a cognate response regulator. Analysis of the genome sequence of S. Dublin identified 31 conserved SK genes. Each SK gene was separately disrupted by lambda Red recombinase-mediated insertion of transposons harbouring unique sequence tags. Calves were challenged with a pool of the mutants together with control strains of defined virulence by the oral and intravenous routes. Quantification of tagged mutants in output pools derived from various tissues and cannulated lymphatic vessels allowed the assignment of spatial roles for each SK following oral inoculation or when the intestinal barrier was bypassed by intravenous delivery. Mutant phenotypes were also assigned in cultured intestinal epithelial cells. Mutants w...Continue Reading

References

Apr 1, 1983·The Journal of Applied Bacteriology·P A Barrow
Sep 1, 1993·Molecular & General Genetics : MGG·A HigashitaniK Horiuchi
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E SheaD W Holden
Mar 7, 1997·Archives of Microbiology·M Bott
Apr 28, 1997·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T Mizuno
Nov 5, 1997·Veterinary Pathology·A J FrostT S Wallis
May 7, 1999·Molecular Microbiology·M HenselB C Berks
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Dec 16, 2000·Epidemiology and Infection·S UzzauJ E Olsen
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·D P ZimmerS Kustu
Jan 5, 2002·Microbes and Infection·C P Lostroh, C A Lee
Jan 23, 2002·Biochimica Et Biophysica Acta·I G JanauschG Unden
Apr 16, 2002·Infection and Immunity·Imke Hansen-Wester, Michael Hensel
May 20, 2003·Molecular Microbiology·Xiuhong FengLinda J Kenney
Aug 20, 2003·Cellular Microbiology·Mark SheppardPietro Mastroeni
Sep 23, 2003·Research in Microbiology·Brit WinnenMilton H Saier
Dec 4, 2003·Journal of Bacteriology·Max TeplitskiBrian M M Ahmer
Nov 4, 2004·Molecular Microbiology·Eirwen MorganTimothy S Wallis
Nov 4, 2004·The Journal of Biological Chemistry·Kaneyoshi YamamotoAkira Ishihama
Jun 14, 2005·Molecular Microbiology·Jetta J E Bijlsma, Eduardo A Groisman
Jun 15, 2005·Cellular Microbiology·Alberto Tierrez, Francisco García-del Portillo
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian K CoombesB Brett Finlay

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Citations

Sep 8, 2012·International Journal of Medical Microbiology : IJMM·Can M UnalKristian Riesbeck
Apr 10, 2012·Research in Veterinary Science·Luciana F CostaRenato L Santos
May 5, 2012·PloS One·Lotte JelsbakJohn Elmerdahl Olsen
Jan 15, 2015·Antimicrobial Agents and Chemotherapy·Tricia A Van LaarKai P Leung
Jun 27, 2019·Journal of Bacteriology·Tamara Renata Machado RibeiroCristiano Gallina Moreira
Mar 23, 2021·Critical Reviews in Microbiology·Lucindo Cardoso de PinaL Caetano M Antunes
Jun 28, 2021·Current Opinion in Microbiology·Mark P Stevens, Robert A Kingsley

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