PMID: 3042748Aug 1, 1988Paper

Role of uhp genes in expression of the Escherichia coli sugar-phosphate transport system

Journal of Bacteriology
L A Weston, R J Kadner

Abstract

The uhpABCT locus of Escherichia coli is responsible for expression of the sugar-phosphate transport system and its induction by external glucose 6-phosphate. Expression of uhpT-lacZ fusions depended on the function of uhpA, uhpB, and uhpC but not of uhpT. A plasmid carrying only uhpT conferred transport activity in a host strain deleted for the uhp region. Thus, uhpT encodes the polypeptide required for transport function, and the other three uhp genes regulate uhpT transcription. The presence of uhpA at elevated copy number resulted in a substantial increase in uhpT expression. This elevated expression was only about 50% of the level seen in induced haploid cells, and no further increase occurred after addition of inducer. Activation by multicopy uhpA was not affected by the status of uhpC but was decreased in the absence of uhpB, suggesting a role for UhpB in directly activating UhpA. Transcription of uhpA, monitored by expression of a uhpA-lacZ fusion, was not affected by either inducer or the presence of the wild-type uhpA allele. The presence of multiple copies of the uhpT promoter region reduced uhpT expression in strains with uhpA in single copy number but not in those with multiple copies, consistent with competition f...Continue Reading

Citations

Dec 2, 1991·International Journal of Cancer. Journal International Du Cancer·C ShalitinE Robinson
Jul 1, 1995·FEMS Microbiology Reviews·K J HellingwerfH V Westerhoff
Mar 11, 2000·European Journal of Biochemistry·G van de WerveA Berteloot
Jan 1, 1993·Journal of Cellular Biochemistry·A J Cozzone
May 27, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Myu YoshidaKaneyoshi Yamamoto
Nov 20, 2012·Nature·Alline R PachecoVanessa Sperandio
Sep 14, 2002·Infection and Immunity·Holger BassoCarlos A Guzmán
Jul 27, 2000·Journal of Bacteriology·Q Chen, R J Kadner
Jan 17, 2020·Bioscience, Biotechnology, and Biochemistry·Tomoyuki TakeyaHiroya Yurimoto
Jun 26, 2007·Journal of Bacteriology·Elizabeth A HussaKaren L Visick
Nov 17, 2020·Frontiers in Microbiology·Vincent CattoirFrançois Guérin

Related Concepts

Bacterial Proteins
Alkalescens-Dispar Group
Genes, Bacterial
Genes, Regulator
Glucosephosphates
Episomes
Early Promoters, Genetic
Homologous Sequences, Nucleic Acid
Sugar Phosphates
Transcription, Genetic

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