Role of uncoupling protein 1 in the anti-obesity effect of beta3-adrenergic agonist in the dog

Research in Veterinary Science
A OmachiMasayuki Saito

Abstract

We have reported that chronic treatment with beta3-adrenoceptor agonists reduces body fat content and induces the expression of mitochondrial thermogenic uncoupling protein 1 (UCP1) in adipose tissue in the dog. To evaluate the role of UCP1 in the anti-obesity effect of the agonists, we isolated adipocytes from subcutaneous fat pad of beagles before and after a 2-week treatment with AJ-9677, a specific beta3-adrenoceptor agonist, and examined their thermogenic activity in vitro. Histological and protein analysis revealed that adipose tissues before the treatment were composed of unilocular cells filled with a single large droplet, while the tissues after the treatment contained many smaller and some multilocular adipocytes expressing UCP1 and abundant mitochondrial proteins. Before the treatment, oxygen consumption rate was very low and did not change even when the cells were stimulated by AJ-9677. Two-week AJ-9677 treatment increased basal oxygen consumption rate by 7-fold, and produced a clear responsiveness to AJ-9677 stimulation. Thus, chronic treatment with AJ-9677 induced UCP1 in adipocytes, where oxygen consumption increased in response to AJ-9677 stimulation. It was suggested that UCP1-dependent energy expenditure in ad...Continue Reading

References

Jul 9, 1991·European Journal of Pharmacology·D LanginM Lafontan
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·O ChampignyB R Holloway
Sep 8, 1989·Science·L J EmorineA D Strosberg
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Feb 8, 2005·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·Katsumi IshiokaMasayuki Saito
Dec 22, 2005·American Journal of Physiology. Endocrinology and Metabolism·Ken-ichi InokumaMasayuki Saito
Dec 5, 2006·Research in Veterinary Science·A OmachiM Saito

❮ Previous
Next ❯

Citations

Sep 15, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Russell T NavenNigel Greene
Jan 5, 2014·PloS One·Yuko Okamatsu-OguraMasayuki Saito
Apr 28, 2009·Veterinary Ophthalmology·Eric C LedbetterJoanne B Messick
Jul 19, 2017·Xenotransplantation·Kefeng YangOuliana Ziouzenkova
Apr 27, 2017·Biochemical and Biophysical Research Communications·Lianjie HouChong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Related Papers

American Journal of Physiology. Endocrinology and Metabolism
Ken-ichi InokumaMasayuki Saito
The Journal of Biological Chemistry
B B Lowell, Eric S Bachman
Cell Metabolism
Shingo KajimuraBruce M Spiegelman
© 2022 Meta ULC. All rights reserved