Role of uncoupling protein 2 (UCP2) expression and 1alpha, 25-dihydroxyvitamin D3 in modulating adipocyte apoptosis

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Xiaocun Sun, Michael B Zemel

Abstract

We previously found that 1alpha, 25-dihydroxyvitamin D3 [1alpha, 25-(OH)2-D3] modulates adipocyte lipid metabolism via a Ca2+-dependent mechanism and inhibits adipocyte UCP2 expression, indicating that the anti-obesity effects of dietary calcium are mediated by suppression of 1alpha, 25-(OH)2-D3 levels. However, because UCP2 reduces mitochondrial potential, we have evaluated the roles of UCP2, mitochondrial uncoupling, and 1alpha, 25-(OH)2-D3 in adipocyte apoptosis. Overexpressing UCP2 in 3T3-L1 cells induced marked reductions in mitochondrial potential (Deltapsi) and ATP production (P<0.01), increases in the expression of caspases (P<0.05), and a decrease in Bcl-2/Bax expression ratio (P<0.01). Physiological doses of 1alpha, 25-(OH)2-D3 (0.1-10 nM) restored mitochondrial Deltapsi in LI-UCP2 cells and protected against UCP2 overexpression-induced apoptosis (P<0.01), whereas a high dose (100 nM) stimulated apoptosis in 3T3-L1 and L1-UCP2 cells (P<0.05). 1alpha, 25-(OH)2-D3 stimulated cytosolic Ca2+ dose-dependently in both 3T3-L1 and L1-UCP2 cells. However, physiological doses suppressed mitochondrial Ca2+ levels by approximately 50% whereas the high dose increased mitochondrial Ca2+ by 25% (P<0.05); this explains stimulation of...Continue Reading

Associated Clinical Trials

References

Sep 1, 1971·The Journal of Pathology·J F Kerr
Feb 1, 1997·Bioscience Reports·T HirschG Kroemer
Jan 23, 1999·Oncogene·G NuñezN Inohara
Jan 29, 2000·FEBS Letters·Y Tsujimoto, S Shimizu
May 16, 2000·The Journal of Biological Chemistry·A G YakovlevM E Smulson
Jul 13, 2000·Cell Calcium·T S MaG J Gallinghouse
Sep 16, 2000·Biochemical Society Symposium·G Kroemer
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H ShiM B Zemel
Jun 1, 2001·European Journal of Immunology·W LiuJ O Liu
Oct 19, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H ShiM B Zemel
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B XueM B Zemel
Dec 14, 2001·The Journal of Biological Chemistry·Leta K NuttStephen G Swisher
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·S S SmailiJ T Russell
Jun 8, 2002·Life Sciences·Tadafumi KatoTakeharu Nagai
Sep 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hang ShiMichael B Zemel
Jan 25, 2003·Cell Calcium·D FerrariR Rizzuto
Feb 4, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S S SmailiR J Youle
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Carmen J NarvaezJoEllen Welsh
May 6, 2003·Biochemical and Biophysical Research Communications·Luca Scorrano, Stanley J Korsmeyer
May 24, 2003·Trends in Molecular Medicine·Mark P Mattson, Guido Kroemer
Aug 6, 2003·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Josef EliasInge Schuster
Nov 25, 2003·Oncogene·Suzanne CoryJerry M Adams
Nov 25, 2003·Oncogene·Rosario RizzutoTullio Pozzan
Dec 4, 2003·Current Opinion in Cell Biology·Tomomi Kuwana, Donald D Newmeyer
Dec 4, 2003·Current Opinion in Cell Biology·Kelly M Boatright, Guy S Salvesen
Dec 11, 2003·Endocrine·Igor N Sergeev, Anthony W Norman
Dec 12, 2003·Development·Damali N Martin, Eric H Baehrecke
Dec 26, 2003·Cell Biochemistry and Function·Lee TantralThottala Jayaraman

❮ Previous
Next ❯

Citations

Jun 28, 2011·European Journal of Nutrition·Silvia Lorente-CebriánMikael Rydén
Dec 5, 2008·Nutrition Reviews·Michael B Zemel, Xiaocun Sun
Jul 4, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·R SakuraiV K Rehan
Jan 1, 2008·Journal of Nutrigenetics and Nutrigenomics·Taru K PilviEero M Mervaala
Dec 22, 2011·Nutrition & Metabolism·Antje Bruckbauer, Michael B Zemel
Dec 4, 2012·Nutrients·Jean-François LandrierFranck Tourniaire
Sep 18, 2007·The Journal of Nutritional Biochemistry·Xiaocun Sun, Michael B Zemel
Jun 15, 2011·Journal of Medicinal Food·Ching-Yi LaiClifton A Baile
Dec 22, 2004·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R B S Harris
Aug 19, 2007·The British Journal of Nutrition·Taru K PilviEero M Mervaala
Apr 1, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·L LiG E Isom
Feb 20, 2008·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·G C MajorA Tremblay
Aug 7, 2009·Clinical Endocrinology·Anna Liza B Valiña-TóthJohn M Flack
Aug 11, 2005·American Journal of Physiology. Endocrinology and Metabolism·M G GnanalinghamT Stephenson
Mar 8, 2006·International Journal of Obesity : Journal of the International Association for the Study of Obesity·X Sun, M B Zemel
Jan 18, 2006·Journal of Biomedical Optics·Andreea Trache, Gerald A Meininger
May 1, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Natalya BodyakPeter M Kang
Feb 16, 2011·Nutrition Research Reviews·Anestis DougkasAnne M Minihane
Mar 12, 2005·Obesity Research·Michael B Zemel
Sep 1, 2005·Obesity Research·Warren G ThompsonMichael B Zemel
Jul 22, 2008·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Angelo Tremblay, Jean-Philippe Chaput
Jan 6, 2010·The Physician and Sportsmedicine·Michael B Zemel
Jan 23, 2014·Molecular Nutrition & Food Research·Igor N Sergeev, Qingming Song
Feb 24, 2017·Reviews in Endocrine & Metabolic Disorders·Silvia SavastanoAnnamaria Colao
May 17, 2012·Nutrition Research Reviews·Qingming Song, Igor N Sergeev
Sep 17, 2020·International Journal of Molecular Sciences·Izabela Szymczak-PajorAgnieszka Śliwińska
May 22, 2008·The Journal of Nutrition·Michael B Zemel, Xiaocun Sun
May 3, 2012·Experimental Biology and Medicine·Shu-Juan YuGuo-Xiong Zhu
Dec 24, 2005·Journal of the American College of Nutrition·Michael B Zemel
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·L TriguerosE Sendra
Mar 28, 2017·The Biochemical Journal·Michael J Berridge
Oct 7, 2020·The American Journal of Clinical Nutrition·Joshua GarfeinEduardo Villamor
Dec 10, 2020·International Journal of Food Sciences and Nutrition·Jee Yeon HongMi Kyung Kim
May 22, 2021·Bone Research·Ruoyu ZhouXianghang Luo
Aug 2, 2021·Archives of Gynecology and Obstetrics·Mina AmiriFahimeh Ramezani Tehrani

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis