Role of urinary cations in the etiology of interstitial cystitis: A multisite study.

International Journal of Urology : Official Journal of the Japanese Urological Association
C Lowell ParsonsPhilip C Bosch

Abstract

To determine whether patients with interstitial cystitis have elevated levels of toxic urinary cations, to identify and quantify these cationic metabolites, and to assess their cytotoxicity. Isolation of cationic fraction was achieved by solid phase extraction using an Oasis MCX cartridge on urine specimens from interstitial cystitis patients and controls. C18 reverse phase high-performance liquid chromatography was used to profile cationic metabolites, and they were quantified by the area under the peaks and normalized to creatinine. Major cationic fraction peaks were identified by reverse phase high-performance liquid chromatography and liquid chromatography-mass spectrometry. HTB-4 urothelial cells were used to determine the cytotoxicity of cationic fraction and of individual metabolites. The reverse phase high-performance liquid chromatography analysis was carried out on cationic fraction metabolites isolated from urine samples of 70 interstitial cystitis patients and 34 controls. The mean for controls versus patients was 3.84 (standard error of the mean 0.20) versus 6.71 (0.37) mAU*min/µg creatinine, respectively (P = 0.0001). The cationic fraction cytotoxicity normalized to creatinine for controls versus patients in mean ...Continue Reading

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Jun 1, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Mahadevan RajasekaranC Lowell Parsons
Oct 20, 2007·The Journal of Urology·C Lowell ParsonsR Nicholas Trotter
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Dec 12, 2013·BJU International·C Lowell ParsonsSulabha Argade
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Mar 31, 2016·World Journal of Urology·Sulabha ArgadeC Lowell Parsons
Oct 3, 2017·Experimental and Therapeutic Medicine·Qixin DuanZhongjie Lu

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Citations

Jul 6, 2021·International Neurourology Journal·Tomohiro UedaNaoki Yoshimura

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