Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†.

Biology of Reproduction
Patrycja KurowskaAgnieszka Rak

Abstract

Vaspin, visceral-adipose-tissue-derived serine protease inhibitor, is involved in the development of obesity, insulin resistance, inflammation, and energy metabolism. Our previous study showed vaspin expression and its regulation in the ovary; however, the role of this adipokine in ovarian cells has never been studied. Here, we studied the in vitro effect of vaspin on various kinase-signaling pathways: mitogen-activated kinase (MAP3/1), serine/threonine kinase (AKT), signal transducer and activator of transcription 3 (STAT3) protein kinase AMP (PRKAA1), protein kinase A (PKA), and on expression of nuclear factor kappa B (NFKB2) as well as on steroid synthesis by porcine ovarian cells. By using western blot, we found that vaspin (1 ng/ml), in a time-dependent manner, increased phosphorylation of MAP3/1, AKT, STAT3, PRKAA1, and PKA, while it decreased the expression of NFKB2. We observed that vaspin, in a dose-dependent manner, increased the basal steroid hormone secretion (progesterone and estradiol), mRNA and protein expression of steroid enzymes using real-time PCR and western blot, respectively, and the mRNA of gonadotropins (FSHR, LHCGR) and steroids (PGR, ESR2) receptors. The stimulatory effect of vaspin on basal steroidoge...Continue Reading

References

Jun 1, 1982·Biology of Reproduction·S StoklosowaC P Channing
Jan 1, 1994·The Journal of Clinical Investigation·Y YoshimuraY Nakamura
Aug 10, 2000·Molecular Biology of the Cell·L ZaliauskieneJ F Collawn
Jul 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kazuyuki HidaYashpal S Kanwar
Dec 13, 2005·Reproductive Toxicology·Andrée M HierlihyChristopher A Price
Mar 4, 2006·Experimental Biology and Medicine·Toshinobu Hatsumi, Yutaka Yamamuro
Nov 10, 2007·Diabetes·Byung-Soo YounMatthias Blüher
Jul 26, 2008·The Journal of International Medical Research·Q LiT Takahashi
Mar 12, 2010·Reproductive Biology and Endocrinology : RB&E·Virginie MaillardJoëlle Dupont
Mar 31, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·D Seto-YoungL Poretsky
Jun 21, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sukanya PhalitakulHideyuki Yamawaki
Mar 27, 2012·Human Reproduction·Maxime ReverchonJoëlle Dupont
May 1, 2012·Gynecologic Oncology·Baigalimaa LuvsandagvaTakashi Minegishi
May 10, 2013·Molecular Biology Reports·Xinsheng LaiHong Chen
May 18, 2013·Reproductive Biology and Endocrinology : RB&E·Agnieszka Rak-MardyłaEwa Lucja Gregoraszczuk
Nov 19, 2013·Journal of Molecular Histology·PengFei LinAiHua Wang
Nov 26, 2013·Molecular Reproduction and Development·Sarah L FieldNicolas M Orsi
Aug 26, 2014·Diabetes Research and Clinical Practice·Rennan FengChanghao Sun
Oct 4, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Wei Qian MmXiaobei Dai
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Mélodie DiotJoëlle Dupont
Jul 15, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Agnieszka RakEwa Łucja Gregoraszczuk
Jun 22, 2016·Scientific Reports·Pawan PuriAnthony J Zeleznik

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Methods Mentioned

BETA
OLETF
electrophoresis
protein assay
dissecting
PCR
ELISA
transgenic

Software Mentioned

GraphPad
PRISM
ImageJ
BLAST

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