Role of vesicle-associated membrane protein 2 in exocytosis of glucagon-like peptide-1 from the murine intestinal L cell

Diabetologia
Samantha K LiP L Brubaker

Abstract

Glucagon-like peptide-1 (GLP-1), secreted by the enteroendocrine L cell, is an incretin hormone that potently stimulates insulin secretion. Although signalling pathways promoting GLP-1 release are well characterised, the mechanisms by which GLP-1-containing granules fuse to the L cell membrane are unknown. As soluble NSF attachment proteins (SNAREs) are known to mediate granule-membrane fusion, the role of vesicle-associated membrane proteins (VAMPs) in GLP-1 exocytosis was examined. SNARE expression was determined in murine GLUTag L cells by RT-PCR and immunoblot and in primary murine L cells by immunofluorescence. Co-immunoprecipitation was used to examine SNARE interactions, while tetanus toxin (TetX)-mediated cleavage of VAMP was used with a GLP-1 secretion assay and total internal reflection fluorescence microscopy to determine the role of VAMP2 in exocytosis. VAMP2 was expressed in murine L cells and localised to secretory granules in GLUTag cells. VAMP1/3 and the core membrane proteins syntaxin1a and synaptosomal-associated protein 25 kDa (SNAP25) were also detected. TetX cleaved VAMPs in GLUTag cells. However, only VAMP2 interacted with syntaxin1a, as did SNAP25 and Munc18-1. TetX treatment of GLUTag cells prevented glu...Continue Reading

References

Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W S TrimbleR H Scheller
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·K PerssonB Ahrén
Aug 15, 2001·American Journal of Physiology. Cell Physiology·X HuangH Y Gaisano
Jan 24, 2003·Diabetes·Younes Anini, Patricia L Brubaker
Jul 23, 2003·The Journal of Biological Chemistry·Laura SheuHerbert Y Gaisano
Aug 16, 2005·Molecular Endocrinology·Beth A Spurlin, Debbie C Thurmond
Dec 13, 2005·Journal of Neurochemistry·Shunsuke UeharaYoshinori Moriyama
May 16, 2007·The Journal of Cell Biology·Mica Ohara-ImaizumiShinya Nagamatsu
Sep 29, 2007·Pharmacology & Therapeutics·Maria Sörhede Winzell, Bo Ahrén
Mar 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Natalia GustavssonWeiping Han
Sep 27, 2008·Endocrinology·Gareth E LimPatricia L Brubaker
Jan 24, 2009·Science·Thomas C Südhof, James E Rothman
Jan 28, 2009·The Journal of Physiology·Natalia GustavssonWeiping Han
May 16, 2009·Nature Reviews. Endocrinology·Julie A Lovshin, Daniel J Drucker
Sep 3, 2009·Cell Metabolism·Charles ThomasKristina Schoonjans
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Svetlana BeglingerChristoph Beglinger
Mar 23, 2010·Endocrinology·Patricia L Brubaker
May 6, 2010·The Journal of Biological Chemistry·Victor S C WongPatricia L Brubaker
Sep 17, 2011·Journal of Pharmacokinetics and Pharmacodynamics·Jonas B MøllerSteen H Ingwersen
Jun 12, 2012·The Journal of Biological Chemistry·Eunjin OhDebbie C Thurmond
Jul 31, 2012·Cell Metabolism·Dan ZhuHerbert Y Gaisano
Aug 2, 2012·The Journal of Clinical Investigation·Emmanuel SommMichel L Aubert
Aug 9, 2012·American Journal of Physiology. Endocrinology and Metabolism·M A PorebaP L Brubaker
Oct 3, 2012·Nature Reviews. Gastroenterology & Hepatology·Charlotte X Dong, Patricia L Brubaker
Feb 21, 2013·Diabetes·Patrick P L LamHerbert Y Gaisano

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Citations

Nov 22, 2014·Diabetes·Jeffrey GagnonPatricia L Brubaker
Sep 15, 2015·International Journal of Endocrinology·XingChun WangShen Qu
Apr 15, 2014·The Journal of Biological Chemistry·Leonardo GuizzettiSavita Dhanvantari
Oct 31, 2019·ELife·Luis F Sifuentes-DominguezEzra Burstein
Mar 7, 2020·Endocrinology·Jhenielle R CampbellPatricia L Brubaker
Feb 9, 2017·The Journal of Biological Chemistry·Nimi VashiDhiraj Kumar
Dec 8, 2015·Clinical Science·Rune E KuhreCamilla Kappe
Sep 22, 2020·Endocrinology·Alexandre MartchenkoPatricia L Brubaker
Jan 16, 2021·Current Opinion in Endocrinology, Diabetes, and Obesity·Ming YangFiona M Gribble
Feb 4, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuta FujiwaraNobuya Inagaki

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