Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation

Journal of Applied Microbiology
Carolina Maldonado Galdeano, Gabriela Perdigón

Abstract

To determine how probiotic bacteria contact with intestinal epithelial and immune cells and the conditions to induce a good mucosal immune stimulation. Lactobacillus casei was studied by transmission electron microscopy (TEM) to determine its interaction with the gut. We compared the influence of viable and nonviable lactic acid bacteria on the intestinal mucosal immune system (IMIS) and their persistence in the gut of mice. TEM showed whole Lact. casei adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp. bulgaricus. For all the strains assayed at 72 h no antigenic particles were found in the intestine. Antigenic particles but not the whole bacteria can enter to epithelial cells and contact with the immune cells. Bacterial viability is a condition for a better stimulation of the IMIS. We demonstrated that only antigenic particle interact with the immune cells and their fast clearance from the gut agrees with those described for the particulate antigens. The regular consumption of probiotics should not adversely affect the host.

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Citations

Sep 25, 2012·Applied Microbiology and Biotechnology·Yueh-Ting TsaiTzu-Ming Pan
Feb 25, 2011·Fish Physiology and Biochemistry·Adolfo JatobáEdemar Roberto Andreatta
Jul 30, 2009·The Journal of Dairy Research·Carolina Maldonado GaldeanoGabriela Perdigón
Apr 21, 2010·Nutrition Research Reviews·Clifford A Adams
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·A SantilloM Albenzio
Apr 10, 2010·Microbiology and Immunology·Heeson Chon, Byungryul Choi
Sep 9, 2005·Clinical and Diagnostic Laboratory Immunology·Gabriel VinderolaGabriela Perdigon
Mar 29, 2013·Clinical and Vaccine Immunology : CVI·Jessica McClemensWaliul I Khan
Aug 3, 2011·BMC Medicine·Girish C DeshpandeSanjay K Patole
Aug 27, 2009·Biologics : Targets & Therapy·Satya Prakash, Aleksandra Malgorzata Urbanska
Jan 1, 2012·Microbial Ecology in Health and Disease·Sampo J Lahtinen
Mar 25, 2014·Applied and Environmental Microbiology·Bai-fen SongLi-jie Tang
Mar 12, 2010·Fish & Shellfish Immunology·S K Nayak
Feb 20, 2007·International Journal of Food Microbiology·Gabriel VinderolaAndrea Quiberoni
Feb 19, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuji Ohashi, Kazunari Ushida
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Jan 1, 2013·ISRN Nutrition·Maria KechagiaEleni Maria Fakiri
Jul 29, 2006·FEMS Immunology and Medical Microbiology·Hanne Risager ChristensenHanne Frøkiaer

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