PMID: 8970997Dec 1, 1996Paper

Role of Vif in human immunodeficiency virus type 1 reverse transcription

Journal of Virology
J GoncalvesD Gabuzda

Abstract

The Vif protein of human immunodeficiency virus type 1 (HIV-1) is important for virion infectivity. Previous studies have shown that vif mutant HIV-1 virions are defective in their ability to synthesize proviral DNA in vivo. Here, we examine the role of Vif in viral DNA synthesis in the endogenous reverse transcriptase (RT) reaction, an in vitro assay in which virions synthesize viral DNA by using endogenous viral RNA as a template. vif mutant virions showed a significant reduction in endogenous RT activity despite similar levels of exogenous RT activity. Analysis of the viral DNA products on agarose gels demonstrated that this reflects reduced synthesis of short minus- and plus-strand DNA products in addition to those of full genomic length. Quantitative PCR analysis of endogenous reverse transcription provided further evidence for reduced formation of both initial and completed reverse transcripts. Vif had no effect on genomic RNA dimerization or the stability of the RNA dimer linkage. These results suggest that Vif is important for an early event after virus entry but preceding or during the early stages of viral DNA synthesis. This may be due to an intrinsic effect on reverse transcription or a preceding postentry event(s),...Continue Reading

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Citations

Nov 9, 2000·Journal of Virology·A Ohagen, D Gabuzda
Aug 9, 2002·Nature·Roger J Pomerantz
Apr 18, 2003·AIDS·Suryaram Gummuluru, Michael Emerman
Oct 7, 2003·Nature Medicine·Mariana MarinDavid Kabat
Oct 19, 2007·Nucleic Acids Research·Yasumasa IwataniJudith G Levin
Jan 13, 2009·The Journal of Biological Chemistry·Jason W RauschStuart F J Le Grice
Apr 21, 2004·Nature Structural & Molecular Biology·Qin YuNathaniel R Landau
Aug 19, 2004·The Biochemical Journal·Reynel CancioGiovanni Maga
Jun 7, 2002·Journal of Virology·Fatima SerhanGhislaine Duisit
Mar 1, 2015·The Journal of Biological Chemistry·Erez PeryDana Gabuzda
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Dec 10, 1998·Nature Medicine·J H SimonM H Malim
Aug 1, 1998·AIDS Research and Human Retroviruses·F Bibollet-RucheM Peeters
Mar 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hal P BogerdBryan R Cullen
Sep 15, 2007·The Journal of Biological Chemistry·Xiao-Yu LiShan Cen
May 4, 2010·The Journal of Microbiology·Su-A LeeSoon-Young Paik
Jan 26, 2010·Expert Reviews in Molecular Medicine·John S Albin, Reuben S Harris
Oct 29, 1998·The Journal of Biological Chemistry·X Yang, D Gabuzda
Aug 21, 2002·The Journal of Experimental Medicine·Jason A LaBonteJoseph Sodroski

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