Role of Viral RNA and Co-opted Cellular ESCRT-I and ESCRT-III Factors in Formation of Tombusvirus Spherules Harboring the Tombusvirus Replicase

Journal of Virology
Nikolay KovalevPeter D Nagy

Abstract

Plus-stranded RNA viruses induce membrane deformations in infected cells in order to build viral replication complexes (VRCs). Tomato bushy stunt virus (TBSV) co-opts cellular ESCRT (endosomal sorting complexes required for transport) proteins to induce the formation of vesicle (spherule)-like structures in the peroxisomal membrane with tight openings toward the cytosol. In this study, using a yeast (Saccharomyces cerevisiae) vps23Δ bro1Δ double-deletion mutant, we showed that the Vps23p ESCRT-I protein (Tsg101 in mammals) and Bro1p (ALIX) ESCRT-associated protein, both of which bind to the viral p33 replication protein, play partially complementary roles in TBSV replication in cells and in cell extracts. Dual expression of dominant-negative versions of Arabidopsis homologs of Vps23p and Bro1p inhibited tombusvirus replication to greater extent than individual expression in Nicotiana benthamiana leaves. We also demonstrated the critical role of Snf7p (CHMP4), Vps20p, and Vps24p ESCRT-III proteins in tombusvirus replication in yeast and in vitro. Electron microscopic imaging of vps23Δ yeast revealed the lack of tombusvirus-induced spherule-like structures, while crescent-like structures are formed in ESCRT-III deletion yeasts re...Continue Reading

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Citations

Jun 3, 2016·Current Opinion in Microbiology·Ana Shulla, Glenn Randall
Mar 21, 2017·Cellular Microbiology·Evan D RossignolEsther Bullitt
Apr 4, 2019·Molecular Plant Pathology·Annette Niehl, Manfred Heinlein
Jan 23, 2019·Frontiers in Microbiology·Katja R Richert-PöggelerRegina G Kleespies
Oct 1, 2019·Annual Review of Virology·Ellen Ketter, Glenn Randall
Oct 17, 2017·Journal of Experimental Botany·Nicolas Pitzalis, Manfred Heinlein
Nov 11, 2019·Nature Reviews. Molecular Cell Biology·Marina VietriHarald Stenmark
Feb 9, 2018·The New Phytologist·Julia Kehr, Friedrich Kragler
Mar 7, 2021·Cells·Arianna CalistriCristina Parolin
Mar 7, 2021·Viruses·María Amelia Sánchez PinaMiguel A Aranda
May 1, 2021·Viruses·Rosalba Rodriguez-PeñaHernan Garcia-Ruiz
Jun 5, 2021·Applied Microbiology and Biotechnology·R Sahaya GlingstonShirisha Nagotu

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