PMID: 7543140Aug 1, 1995Paper

Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-1

The Journal of Experimental Medicine
M SaifuddinG T Spear

Abstract

This study investigates whether cell-derived glycosylphosphatidylinositol-linked complement control proteins CD55 and CD59 can be incorporated into HIV-1 virions and contribute to complement resistance. Virus was prepared by transfection of cell lines with pNL4-3, and primary isolates of HIV-1 were derived from patients' PBMCs. Virus was tested for sensitivity to complement-mediated virolysis in the presence of anti-gp160 antibody. Viral preparations from JY33 cells, which lack CD55 and CD59, were highly sensitive to complement. HIV-1 preparations from H9 and U937 cells, which express low levels of CD55 and CD59, had intermediate to high sensitivity while other cell line-derived viruses and primary isolates of HIV-1 were resistant to complement-mediated virolysis. Although the primary isolates were not lysed, they activated complement as measured by binding to a complement receptor positive cell line. While the primary isolates were resistant to lysis in the presence of HIV-specific antibody, antibody to CD59 induced lysis. Likewise, antibody to CD55 and CD59 induced lysis of cell line-derived virus. Western blot analysis of purified virus showed bands corresponding to CD55 and CD59. Phosphatidylinositol-specific phospholipase ...Continue Reading

References

Oct 16, 1975·Nature·R M WelshM B Oldstone
Mar 1, 1978·The Journal of Experimental Medicine·R M BartholomewH J Müller-Eberhard
Sep 1, 1991·Immunology Today·P J Lachmann
Jan 1, 1989·Annual Review of Immunology·D M Lublin, J P Atkinson
Aug 1, 1989·The Journal of Clinical Investigation·T McNearneyJ P Atkinson
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M E MedofV Nussenzweig
Jan 1, 1988·Annual Review of Biochemistry·M A Ferguson, A F Williams
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YoshidaY Hinuma
Nov 1, 1993·AIDS Research and Human Retroviruses·R J Orentas, J E Hildreth

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Citations

Jan 1, 1997·Springer Seminars in Immunopathology·D C Montefiori
May 8, 2003·Journal of Biosciences·John BernetArvind Sahu
Feb 1, 1996·Research in Immunology·J P Atkinson
Aug 26, 1998·Immunology Today·M J TremblayR Cantin
May 24, 2003·Vaccine·Heribert StoiberManfred P Dierich
Mar 17, 2010·Cellular & Molecular Immunology·Qigui YuXuebin Qin
Feb 1, 1996·Nature Medicine·M P DierichA Clivio
Aug 10, 2000·Clinical and Experimental Immunology·M SaifuddinK A Roebuck
Apr 5, 2001·The New England Journal of Medicine·M J Walport
Oct 30, 2003·Viral Immunology·Yen-Ting ChenShan-Ling Hung
Dec 1, 1995·AIDS Research and Human Retroviruses·D C Montefiori
Feb 1, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·P MarschangM P Dierich
Mar 12, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M SaifuddinG T Spear
Jan 7, 1998·Immunological Reviews·C SpethM P Dierich
Oct 19, 2012·Journal of Virology·Gülşen Özkaya ŞahinMarianne Jansson
May 14, 2005·Journal of Virology·Réjean CantinMichel J Tremblay

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