Role of wzxE in Salmonella Typhimurium lipopolysaccharide biosynthesis and interleukin-8 secretion regulation in human intestinal epithelial cells.

Microbiological Research
Ke-Chuan WangShiuh-Bin Fang

Abstract

In Salmonella Typhimurium (S. Typhimurium), lipopolysaccharide (LPS) anchored on the bacterial outer membrane is a major immune stimulus that can broadly activate immune cells and induce innate immune responses. wzxE is involved in bacterial LPS biosynthesis but has rarely been reported in Salmonella; wzxE encodes a flipase that can flip the precursor of LPS across the membrane into the periplasm space. Our preliminary data showed that the wzxE transposon mutant of S. Typhimurium could not significantly adhere to and invade into HEp-2 cells, but the mechanism remains unknown. In this study, we infected human LS174T, Caco-2, HeLa, and THP-1 cells with the wild-type S. Typhimurium strain SL1344, its wzxE mutant, and its complemented strain. wzxE depletion significantly attenuated bacterial adhesion and internalization in the four cell types. In addition, the postinfectious production of interleukin-8 (IL-8) was significantly decreased in the Caco-2 cells infected with the wzxE mutant. Bacterial LPS stained with polymyxin B probe also exhibited a reduced signal in the wzxE mutant. The silver staining of purified LPS demonstrated a significant reduction of the O-antigen (OAg) chain in the wzxE mutant. To confirm the role of OAg in ...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Jun 1, 1989·Journal of Leukocyte Biology·K M TrotterD B Lause
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Sep 1, 1993·Microbiological Reviews·C A Schnaitman, J D Klena
May 1, 1995·Trends in Microbiology·C Whitfield
Jan 1, 1993·Journal of Bacteriology·S K CollinsonW W Kay
Jun 17, 1998·FEMS Microbiology Letters·J W AustinS K Collinson
Nov 26, 1998·The Journal of Biological Chemistry·T J WyckoffC R Raetz
Apr 4, 2002·Molecular Microbiology·Cristina SolanoIñigo Lasa
May 15, 2002·FEMS Microbiology Reviews·Inge Lerouge, Jos Vanderleyden
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Nov 1, 2002·Journal of Clinical Microbiology·Li Tan, Parwinder S Grewal
Sep 23, 2003·Infection and Immunity·Simon ClareGordon Dougan
Oct 6, 2005·Biochemical and Biophysical Research Communications·Sang Kil LeeWon Ho Kim
Jan 30, 2007·Research in Microbiology·Silvia Herrera-LeónMaria Aurora Echeita
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop
Oct 17, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yoshihiko SawaShigemitsu Yoshida
Aug 19, 2008·FEMS Microbiology Letters·Monica M Cunneen, Peter R Reeves
Apr 25, 2009·Pediatric Research·Eva LevyMarietta Xanthou
May 7, 2009·Biochimica Et Biophysica Acta·Paola SperandeoAlessandra Polissi
Nov 21, 2009·Glycobiology·Magnus LundborgGöran Widmalm
Nov 15, 2011·Cell·Ivan ZanoniJonathan C Kagan
Sep 29, 2012·Environmental Microbiology·Salim T Islam, Joseph S Lam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.