Role of YadA in resistance to killing of Yersinia enterocolitica by antimicrobial polypeptides of human granulocytes.

Infection and Immunity
L G VisserR van Furth

Abstract

The virulence plasmid pYVe of Yersinia enterocolitica codes for the production of the outer membrane protein YadA and the secretion of several proteins, called Yops, which may protect this bacterium against killing by human granulocytes. Granulocytes kill ingested microorganisms by oxygen-dependent and oxygen-independent mechanisms, the latter including antimicrobial polypeptides. The aim of this study was to determine whether virulent (pYVe+) Y. enterocolitica and plasmid-cured avirulent (pYVe-) Y. enterocolitica differ in susceptibility to antimicrobial polypeptides extracted from granules of human granulocytes. The acetic acid granule extract contained several polypeptides with antimicrobial activity against Y. enterocolitica as determined by gel overlay and radial diffusion assays. Two of these polypeptides were identified as lysozyme and defensins. pYVe+ Y. enterocolitica was less susceptible than pYVe- Y. enterocolitica to the antimicrobial activity of granule extract, lysozyme, and defensins as determined in a suspension assay, which indicated that the pYVe plasmid mediates a reduced susceptibility to these polypeptides. The role of YadA in the resistance to antimicrobial polypeptides was analyzed by using mutants of Y. ...Continue Reading

References

Jan 1, 1992·Medical Microbiology and Immunology·T GanzR I Lehrer
Sep 1, 1992·Infection and Immunity·P B EisenhauerR I Lehrer
Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Mar 21, 1991·Journal of Immunological Methods·R I LehrerP Eisenhauer
Dec 1, 1990·Infection and Immunity·P EisenhauerR I Lehrer
Aug 1, 1989·The Journal of Clinical Investigation·R I LehrerM E Selsted
Oct 1, 1989·Molecular Microbiology·G R CornelisJ C Vanooteghem
Jan 1, 1987·Reviews of Infectious Diseases·G CornelisG Wauters
May 1, 1987·Infection and Immunity·C J LianC H Pai
Jan 1, 1984·Contemporary Topics in Immunobiology·J K Spitznagel
May 1, 1984·Infection and Immunity·R V Lachica, D L Zink
Jan 1, 1993·Annual Review of Immunology·R I LehrerT Ganz

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Citations

Jun 19, 2003·Journal of Bacteriology·Andreas RoggenkampJürgen Heesemann
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Dec 21, 2010·PloS One·Salome Casutt-MeyerGuy R Cornelis
Nov 24, 2004·Infection and Immunity·Yvonne SchmidErwin Bohn
Jul 16, 2002·Infection and Immunity·Nadine GrosdentGuy R Cornelis
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·G R CornelisI Stainier

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