Role of YiaX2 in L-ascorbate transport in Klebsiella pneumoniae 13882

Canadian Journal of Microbiology
Evangelina CamposJosefa Badia

Abstract

The yiaK-S operon is required for aerobic growth on L-ascorbate in several Enterobacteriaceae. Here we present evidence that the yiaX2 gene belonging to the yiaK-S operon of Klebsiella pneumoniae 13882, which encodes a protein similar to the putative transporters classified as the major facilitator superfamily, is involved in the uptake of L-ascorbate. Concentration kinetic analysis yielded an apparent K(m) of YiaX2 for L-ascorbate of 161.38 +/-8.28 micromol x L(-1) and a Vmax of 3.81 +/- 0.60 nmol x mg(-1) x min(-1). This carrier uses the energy from electrochemical gradients, since it was inhibited by carbonyl cyanide m-chlorophenylhydrazone, a hydrophobic proton conductor that dissipates proton motive force.

References

Feb 19, 1998·Microbiology·Henrik ChartBernard Rowe
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·S S PaoM H Saier
Mar 20, 2003·Journal of Bacteriology·Zhongge ZhangMilton H Saier
Oct 18, 2003·Journal of Bacteriology·Rosa GimenezLaura Baldoma
Dec 12, 2003·Molecular Membrane Biology·Titia H PlantingaArnold J M Driessen
Oct 2, 2007·Microbiology·Evangelina CamposJosefa Badia
Dec 6, 2008·BMC Genomics·Manisha GaurRajendra Prasad

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