Roles for Srp72p in assembly, nuclear export and function of the signal recognition particle.

RNA Biology
Rob W van NuesJeremy D Brown

Abstract

Co-translational protein targeting to the endoplasmic reticulum is catalysed by the signal recognition particle, a conserved ribonucleoprotein. Key activities of SRP--signal sequence binding, and inhibition of ribosomal translation elongation--require interactions of SRP with distant locations on the ribosome. A heterodimer of Srp72p and Srp68p localise to the central portion of the SRP complex, and may co-ordinate its activities. A series of mutations within Srp72p were examined individually, in combination and in the presence of mutations within SRP RNA. In this analysis mutations within Srp72p fell into two groups, identifying separate interactions/functions of the protein. Much of Srp72p is predicted to be alpha helical tetratricopeptide repeat motifs, with the C-terminus forming a separate unstructured region. Mutations towards the C-terminal end of the alpha helical region reveal a specific genetic interaction with a conserved motif in the central helix of SRP RNA. In contrast, mutations within the C-terminal region of Srp72p have genetic interactions across the RNA. Many mutant combinations impaired function rather than inhibiting assembly of SRP. However, one specific combination of Srp72p and SRP RNA mutations led to a...Continue Reading

Citations

Aug 28, 2014·Nucleic Acids Research·Eileen LeungJeremy D Brown
Nov 1, 2009·Reumatología clinica·Mónica Vázquez Del Mercado EspinosaJorge Aguilar Arreola
Aug 21, 2009·Protein Science : a Publication of the Protein Society·Elena IakhiaevaChristian Zwieb
Feb 19, 2015·RNA Biology·Klemens Wild, Irmgard Sinning
Apr 4, 2017·Journal of Molecular Cell Biology·Yina GaoZhongzhou Chen
Jun 4, 2019·Journal of Molecular Cell Biology·Hendrik TäuberMarcel Köhn
Aug 14, 2019·Biological Chemistry·Klemens WildIrmgard Sinning
Aug 12, 2014·Molecular Medicine Reports·Hailong WangRui Guo

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