Roles of a conserved proline in the internal fusion peptide of Ebola glycoprotein

FEBS Letters
María J GómaraJ L Nieva

Abstract

The structural determinants underlying the functionality of viral internal fusion peptides (IFPs) are not well understood. We have compared EBOwt (GAAIGLAWIPYFGPAAE), representing the IFP of the Ebola fusion protein GP, and EBOwt (GAAIGLAWIPYFGRAAE) derived from a non-functional mutant with conserved Pro537 substituted by Arg. P537R substitution did not abrogate peptide-membrane association, but interfered with the ability to induce bilayer destabilization. Structural determinations suggest that Pro537 is required to preserve a membrane-perturbing local conformation in apolar environments.

References

Jan 1, 1990·Annual Review of Physiology·J M White
Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
Oct 1, 1969·Biochemistry·J Eisinger
Feb 23, 1996·The Journal of Biological Chemistry·E Pérez-PayáS E Blondelle
Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
Sep 29, 1998·Methods in Enzymology·S H WhiteK Hristova
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·V N MalashkevichP S Kim
May 18, 1999·Journal of Molecular Biology·M MonnéG von Heijne
Sep 2, 1999·Archives of Virology. Supplementum·H FeldmannH D Klenk
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Sep 6, 2000·Trends in Biochemical Sciences·J A Killian, G von Heijne
Jul 4, 2001·The Journal of Biological Chemistry·S H WhiteK Hristova
Nov 21, 2002·Cellular and Molecular Life Sciences : CMLS·G Basañez
Jul 23, 2003·Biochimica Et Biophysica Acta·José L Nieva, Aitziber Agirre
Dec 16, 2003·Journal of Molecular Biology·Mar OrzáezIsmael Mingarro

❮ Previous
Next ❯

Citations

Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sonia M GregoryLukas K Tamm
Jan 1, 2009·Future Virology·Jeffrey E Lee, Erica Ollmann Saphire
Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Apr 8, 2014·Chemistry and Physics of Lipids·Beatriz ApellánizJosé L Nieva
Jul 15, 2015·Cell Cycle·Qian CongNick V Grishin
Oct 10, 2006·Virology·Matthew L PlassmeyerFrancisco González-Scarano
Sep 24, 2013·Biochimica Et Biophysica Acta·Audrey Agopian, Sabine Castano
Jun 21, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Judit SzécsiEls Verhoeyen
Jan 31, 2006·Expert Review of Anti-infective Therapy·Jason Paragas, Thomas W Geisbert
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kelly L WarfieldSina Bavari
Apr 4, 2012·Viruses·Catherine L HuntWendy Maury
Mar 5, 2021·Journal of Structural Biology·Debnath Pal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.