Abstract
Three transmembrane glutamic acid residues play essential roles in the metal-tetracycline/H+ antiporter Tet(K) of Staphylococcus aureus [Fujihira et al., FEBS Lett. 391 (1996) 243-246]. In the putative hydrophilic loop region of the Tet(K) and Tet(L) proteins, six acidic residues are conserved. Asp74, Asp200, Asp318 and Glu381 are located on the putative cytoplasmic side, and Asp39 and Glu345 on the putative periplasmic side. These residues were replaced by a neutral amino acid residue or a charge-conserved one. In contrast to the transmembrane glutamic acid residues, the replacement of the two glutamic acid residues (Glu345 and Glu381) did not affect the tetracycline resistance level. Out of the other four aspartic acid residues, the only essential residue is Asp318, any replacement of which resulted in complete loss of the tetracycline resistance and transport activity. Asp318 is located in cytoplasmic loop 10-11 in the putative 14-transmembrane-segment topology of Tet(K). In the case of the tetracycline exporters of Gram-negative bacteria, the only essential acidic residue in the cytoplasmic loop region is located in loop 2-3 [Yamaguchi et al., Biochemistry 31 (1992) 8344-8348]. It may be a general role for tetracycline effl...Continue Reading
References
Sep 8, 1992·Biochemistry·A YamaguchiT Sawai
Apr 1, 1992·Antimicrobial Agents and Chemotherapy·S B Levy
Mar 1, 1992·Antimicrobial Agents and Chemotherapy·S SchwarzH C Wegener
May 6, 1991·FEBS Letters·A YamaguchiT Sawai
Aug 1, 1990·Journal of Bioenergetics and Biomembranes·P J Henderson
Jan 1, 1990·Antimicrobial Agents and Chemotherapy·E K ManavathuD E Taylor
Jan 1, 1985·Gene·T HoshinoK Furukawa
Feb 1, 1986·Journal of Bacteriology·V Burdett
Dec 1, 1988·Journal of Bacteriology·M Mojumdar, S A Khan
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Oct 1, 1987·Antimicrobial Agents and Chemotherapy·L M McMurryS B Levy
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Feb 1, 1981·Journal of Bacteriology·T YamamotoS Yamagishi
Nov 1, 1983·Plasmid·S A Khan, R P Novick
Aug 1, 1995·Journal of Bacteriology·A A Guffanti, T A Krulwich
May 29, 1995·FEBS Letters·A YamaguchiM Sasatsu
Feb 1, 1994·Biological & Pharmaceutical Bulletin·N NoguchiM Kono
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·I T PaulsenR A Skurray
Aug 12, 1996·FEBS Letters·E FujihiraA Yamaguchi
Jun 1, 1997·Journal of Bacteriology·S L GinnR A Skurray
Jul 28, 1997·FEBS Letters·T HirataA Yamaguchi
Citations
Sep 21, 2005·Biochemistry·Magdia De JesusTerry A Krulwich
Mar 2, 2002·Journal of Bacteriology·Jie Jin, Terry A Krulwich
Jun 26, 2007·Journal of Molecular Microbiology and Biotechnology·Karl A HassanMelissa H Brown
Nov 26, 2015·Antimicrobial Agents and Chemotherapy·Marius LinkeviciusDan I Andersson
Oct 28, 1999·Microbiology·T L Bannam, J I Rood
Oct 3, 1998·The Journal of Biological Chemistry·A A GuffantiT A Krulwich
Dec 21, 2006·Biochemistry·Karl A HassanMelissa H Brown