Roles of aconitase in growth, metabolism, and morphological differentiation of Streptomyces coelicolor

Journal of Bacteriology
P H ViollierC J Thompson

Abstract

The studies of aconitase presented here, along with those of citrate synthase (P. H. Viollier, W. Minas, G. E. Dale, M. Folcher, and C. J. Thompson, J. Bacteriol. 183:3184-3192, 2001), were undertaken to investigate the role of the tricarboxylic acid (TCA) cycle in Streptomyces coelicolor development. A single aconitase activity (AcoA) was detected in protein extracts of cultures during column purification. The deduced amino acid sequence of the cloned acoA gene constituted the N-terminal sequence of semipurified AcoA and was homologous to bacterial A-type aconitases and bifunctional eukaryotic aconitases (iron regulatory proteins). The fact that an acoA disruption mutant (BZ4) did not grow on minimal glucose media in the absence of glutamate confirmed that this gene encoded the primary vegetative aconitase catalyzing flux through the TCA cycle. On glucose-based complete medium, BZ4 had defects in growth, antibiotic biosynthesis, and aerial hypha formation, partially due to medium acidification and accumulation of citrate. The inhibitory effects of acids and citrate on BZ4 were partly suppressed by buffer or by introducing a citrate synthase mutation. However, the fact that growth of an acoA citA mutant remained impaired, even ...Continue Reading

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Citations

Jun 27, 2012·Antonie van Leeuwenhoek·Elodie TenconiSébastien Rigali
Dec 4, 2001·Current Opinion in Microbiology·K F Chater
May 21, 2005·Biotechnology Progress·Wilfried WeberMartin Fussenegger
Apr 23, 2013·Nucleic Acids Research·Aline Tabib-SalazarMark S Paget
Oct 13, 2009·Journal of Bacteriology·Uriel KoziolFrancisco Noya
Dec 11, 2007·Journal of Bacteriology·Nadria D GordonAmy M Gehring
Nov 24, 2011·Archaea : an International Microbiological Journal·Andrew I Yao, Marc T Facciotti
Jan 15, 2014·Applied Microbiology and Biotechnology·Tiago BeitesMarta V Mendes
Jan 2, 2007·Journal of Biotechnology·Alexander KernAnton Glieder
Jan 20, 2006·Molecular Microbiology·Joanne M WilleyJustin R Nodwell
Jul 22, 2014·Molecular Microbiology·Elaine R Frawley, Ferric C Fang
Dec 17, 2014·The Biochemical Journal·Hao HuangAnne Grove
Aug 17, 2006·FEMS Microbiology Reviews·Keith F Chater, Govind Chandra
Apr 28, 2011·RNA Biology·Michael-Paul VockenhuberBeatrix Suess
Mar 5, 2015·Metallomics : Integrated Biometal Science·Halie K Miller, Victoria Auerbuch
Jul 9, 2008·The Journal of Biological Chemistry·Irina BorodinaJens Nielsen
Jun 25, 2018·Archives of Microbiology·Tóshiko Takahashi-ÍñiguezMaría Elena Flores
Nov 3, 2012·Environmental Microbiology·Ewelina MichtaYvonne Mast
Sep 10, 2015·Microbiology Spectrum·Fabian M Commichau, Jörg Stülke

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