Roles of B739_1343 in iron acquisition and pathogenesis in Riemerella anatipestifer CH-1 and evaluation of the RA-CH-1ΔB739_1343 mutant as an attenuated vaccine

PloS One
MaFeng LiuAn-Chun Cheng

Abstract

Iron is one of the most important elements for bacterial survival and pathogenicity. The iron uptake mechanism of Riemerella anatipestifer (R. anatipestifer, RA), a major pathogen that causes septicemia and polyserositis in ducks, is largely unknown. Here, the functions of the putative TonB-dependent iron transporter of RA-CH-1, B739_1343, in iron utilization and pathogenicity were investigated. Under iron-starved conditions, the mutant strain RA-CH-1ΔB739_1343 exhibited more seriously impaired growth than the wild-type strain RA-CH-1, and the expression of B739_1343 in the mutant strain restored growth. qRT-PCR results showed that the transcription of B739_1343 was not regulated by iron conditions. In an animal model, the median lethal dose (LD50) of the mutant strain RA-CH-1ΔB739_1343 increased more than 104-fold (1.6×1012 CFU) compared to that of the wild-type strain RA-CH-1 (1.43×108 CFU). In a duck co-infection model, the mutant strain RA-CH-1ΔB739_1343 was outcompeted by the wild-type RA-CH-1 in the blood, liver and brain of infected ducks, indicating that B739_1343 is a virulence factor of RA-CH-1. Finally, immunization with live bacteria of the mutant strain RA-CH-1ΔB739_1343 protected 83.33% of ducks against a high-dos...Continue Reading

References

Sep 28, 1977·Archives of Microbiology·R E HancockV Braun
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Shelley M PayneNicola M L Davies
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Mar 1, 1995·Avian Pathology : Journal of the W.V.P.A·P PathanasophonT Tanticharoenyos
Jun 1, 1991·Avian Pathology : Journal of the W.V.P.A·T S Sandhu, M L Leister
Jun 24, 2010·BMC Microbiology·Robert J WatsonDavid J Clarke
Dec 17, 2010·Immunologic Research·Bobby J Cherayil
Feb 1, 2011·Current Opinion in Chemical Biology·Volkmar Braun, Klaus Hantke
May 14, 2011·Metallomics : Integrated Biometal Science·Pierre CornelisTiffany Vinckx
Oct 22, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Shreya Datta, Jorge H Crosa
May 21, 2013·Cell Host & Microbe·James E Cassat, Eric P Skaar
Feb 11, 2014·Pakistan Journal of Biological Sciences : PJBS·Kuldeep DhamaShambhu Dayal Singh
May 2, 2014·Current Opinion in Chemical Biology·Heidi ContrerasCelia W Goulding
Apr 19, 2015·Veterinary Microbiology·Gaëlle Porcheron, Charles M Dozois
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg

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Methods Mentioned

BETA
RNA-Seq
ELISA
PCR
Protein Assay

Software Mentioned

Clustal W2
Phyre
SPSS statistics
BLAST
GraphPad Prism

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