Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct.

American Journal of Physiology. Renal Physiology
Chung-Lin ChouMark A Knepper

Abstract

Collecting duct cells swell when exposed to arginine vasopressin (AVP) in the presence of a transepithelial osmolality gradient. We investigated the mechanisms of AVP-induced cell swelling in isolated, perfused rat inner medullary collecting ducts (IMCDs) using quantitative video microscopy and fluorescence-based measurements of transepithelial water transport. We tested the roles of transepithelial water flow, basolateral solute entry, and the cytoskeleton (actomyosin). When a transepithelial osmolality gradient was imposed by addition of NaCl to the bath, AVP significantly increased both water flux and cell height. When the osmolality gradient was imposed by addition of mannitol, AVP increased water flux but not cell height, suggesting that AVP-induced cell swelling requires a NaCl gradient and is not merely dependent on the associated water flux. Bumetanide (Na-K-2Cl cotransporter inhibitor) added to the bath markedly diminished the AVP-induced cell height increase. AVP-induced cell swelling was absent in IMCDs from NKCC1-knockout mice. In rat IMCDs, replacement of Na, K, or Cl in the peritubular bath caused significant cell shrinkage, consistent with a basolateral solute transport pathway dependent on all three ions. Immuno...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·S M WallM A Knepper
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Jun 1, 1989·Pflügers Archiv : European journal of physiology·R W Grunewald, R K Kinne
Aug 1, 1989·Kidney International·C GruppR K Kinne
Jan 1, 1987·The Journal of Membrane Biology·K Strange, K R Spring
Sep 1, 1986·The American Journal of Physiology·J M SandsK R Spring
Dec 1, 1973·The Journal of Clinical Investigation·P B Woodhall, C C Tisher
Oct 1, 1995·The American Journal of Physiology·S M WallK E Woodward
Dec 1, 1996·Journal of the American Society of Nephrology : JASN·S M GinnsJ B Wade
Mar 5, 1998·The American Journal of Physiology·F M FaraciD D Heistad
Nov 14, 1998·The American Journal of Physiology·S CaiP De Lanerolle
Jun 1, 2000·The Journal of Biological Chemistry·R L EvansJ E Melvin
Feb 22, 2001·Journal of the American Society of Nephrology : JASN·Mika IkebeKimio Tomita
Apr 9, 2001·American Journal of Physiology. Renal Physiology·S M WallS J Park
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Caterina Di CianoAndrás Kapus
Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
Feb 16, 2005·The Journal of Biological Chemistry·Jason D HoffertMark A Knepper
Aug 11, 2006·Molecular & Cellular Proteomics : MCP·Ming-Jiun YuMark A Knepper
Feb 10, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E K HoffmannW S Marshall

❮ Previous
Next ❯

Citations

Apr 25, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Shiho WakamatsuKimio Tomita
Sep 24, 2011·The Journal of Biological Chemistry·Liudmila ErokhovaPeter Pohl
Feb 6, 2009·The Journal of Biological Chemistry·Jason D HoffertMark A Knepper
Jul 4, 2008·American Journal of Physiology. Cell Physiology·Ming-Jiun YuMark A Knepper
Jul 1, 2011·American Journal of Physiology. Renal Physiology·Volker Vallon, Timo Rieg
Apr 29, 2014·Bulletin of Experimental Biology and Medicine·A V Babina, V A Lavrinenko
Feb 13, 2010·Annual Review of Physiology·Helle A Praetorius, Jens Leipziger
Apr 16, 2013·Clinical and Experimental Nephrology·Justin L L WilsonMark A Knepper
Mar 30, 2016·Pflügers Archiv : European journal of physiology·Joana Raquel MartinsSophie Demolombe
Jun 4, 2013·Mathematical Biosciences·Sotirios G ZarogiannisEvgeniy I Solenov
Jan 22, 2017·Physiological Reports·Ezigbobiara N UmejiegoChung-Lin Chou
Jul 25, 2014·American Journal of Physiology. Renal Physiology·Akshay SanghiMark A Knepper
Jan 7, 2020·American Journal of Physiology. Renal Physiology·Kiyoshi IsobeMark A Knepper
Dec 29, 2020·Physiological Reports·Nathaniel J HimmelMitsi A Blount

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.