Roles of cAMP in regulation of both MAP kinase and p34(cdc2) kinase activity during meiotic progression, especially beyond the MI stage

Molecular Reproduction and Development
Masayuki Shimada, Takato Terada

Abstract

Time-dependent changes in the level of adenosine cyclic AMP (cAMP) in porcine oocytes during meiotic progression from the germinal vesicle stage (GV stage) to the metaphase II stage (MII stage) were examined using reversed-phase HPLC with UV detection. The concentration of cAMP in oocytes reached a peak at 8 hr of cultivation of cumulus-oocyte complexes (COCs), but it was dramatically decreased after 12-hr cultivation. After a 28-hr cultivation period, the level of cAMP in the oocytes had significantly reduced further, and the basal level of cAMP was observed in oocytes cultured at 32 hr and for up to 48 hr. When phosphatidylinositol 3-kinase (PI 3-kinase) or protein kinase C (PKC) in cumulus cells [which were required for meiotic progression to the MII stage in porcine oocytes (Shimada and Terada, 2001: Biol Reprod 64:1106-1114)] was suppressed by each specific inhibitor following initial 24-hr cultivation of COCs, cAMP level in the oocytes was significantly increased. After 24-hr cultivation in the maturation medium, COCs, which were cultured for an additional 24 hr in the presence of either forskolin or 3-isobutyl-1-methylxanthine (IBMX), exhibited a significant increase in the oocyte cAMP level to the similar level of that ...Continue Reading

References

Oct 1, 1985·Biology of Reproduction·E A Bornslaeger, R M Schultz
Jul 1, 1987·Developmental Biology·W J LarsenG D Brunner
Apr 1, 1981·The Journal of Experimental Zoology·R M Moor, J P Heslop
Jul 1, 1994·Molecular and Cellular Biology·W MattenG F Vande Woude
Mar 1, 1994·Journal of Reproduction and Fertility·M MattioliE Seren
Aug 1, 1995·Zygote : the Biology of Gametes and Early Embryos·M InoueE Sato

❮ Previous
Next ❯

Citations

Sep 12, 2007·Journal of Assisted Reproduction and Genetics·Junichi HasegawaTakashi Okai
Nov 8, 2003·Molecular and Cellular Endocrinology·Tetsuji OkazakiMasayuki Shimada
May 16, 2009·Molecular Human Reproduction·Meijia ZhangGuoliang Xia
Aug 19, 2007·The Journal of Reproduction and Development·Marie MorikawaKunihiko Naito
May 1, 2009·The Journal of Reproduction and Development·Hong-Thuy BuiTakashi Miyano
Feb 18, 2004·The Journal of Reproduction and Development·Qing-Yuan Sun, Takashi Nagai
Feb 19, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Junya ItoNaomi Kashiwazaki
Apr 5, 2016·Reproduction in Domestic Animals = Zuchthygiene·D TöpferA C Spannbrucker
Jul 31, 2018·Reproduction in Domestic Animals = Zuchthygiene·Mahmoud MoussaJiang-Hua Shang
Feb 10, 2007·Molecular Reproduction and Development·Sylvie Bilodeau-GoeseelsFrançois J Richard
Apr 22, 2009·Molecular Reproduction and Development·Radek ProcházkaAlzbeta Mlynarcíková
Mar 24, 2006·Reproduction, Fertility, and Development·Christopher G GrupenDavid T Armstrong
Jan 19, 2013·Reproduction, Fertility, and Development·Jason R Herrick
May 12, 2012·Reproductive Medicine and Biology·Masayuki Shimada
Jan 20, 2011·Reproductive Medicine and Biology·Ilse Silvia Cayo-ColcaTakashi Miyano

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.