Roles of CD4+ and CD8+ T cells in adjuvant activity of diesel exhaust particles in mice

International Archives of Allergy and Immunology
Hidekazu FujimakiT Endo

Abstract

Through an imbalance in Th1 and Th2 cytokine profiles, diesel exhaust particles (DEP) are thought to induce Th2-dominated IgE and IgG1 production. However, the roles of CD4+ and CD8+ T-cell subtypes in the increased immune responses to antigen in mice exposed to DEP are unclear. In the present study, we investigated whether treatment with anti-CD4 or anti-CD8 mAb abrogated the adjuvant activity of DEP. On day -1 and day 1, each group of mice was injected intraperitoneally with anti-CD4, anti-CD8, or rat IgG (vehicle). On day 0, the mice were immunized with ovalbumin (OVA) or OVA plus DEP. After 3 weeks, each mouse was boosted with 10 microg of OVA alone. On day 7 after the first injection with OVA+DEP or OVA alone, the numbers of total, IA+, CD80+/IA+ and CD86+/IA+ cells in peritoneal exudate cells (PEC) were higher in OVA+DEP-immunized mice than in OVA-immunized mice. Depletion of CD8+ cells resulted in a modulation of the production of granulocyte-macrophage colony-stimulating factor, IL-12 and PGE(2) in peritoneal exudate fluid from OVA+DEP-immunized mice. On day 28, DEP injection markedly increased IL-4 production in the culture supernatants of spleen cells from CD4+ or CD8+-depleted mice. Depletion of CD8+ cells in OVA+DEP...Continue Reading

Citations

Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·H FujimakiT Endo
May 2, 2003·American Journal of Respiratory and Critical Care Medicine·Stephanie SénéchalBenoît Wallaert
Jun 19, 2016·The Journal of Allergy and Clinical Immunology·Katrien C De GroveGuy F Joos
May 18, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K C De GroveT Maes
Aug 30, 2008·Immunopharmacology and Immunotoxicology·Ken-Ichiro InoueHirohisa Takano
Nov 12, 2015·Frontiers in Physiology·Lei NiLi Zuo
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Junxiang Wan, David Diaz-Sanchez

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