Roles of COX-2 and iNOS in the bony repair of the injured growth plate cartilage

Journal of Cellular Biochemistry
G ArasapamC J Xian

Abstract

Growth plate injuries often lead to bone growth defects, which primarily occur due to bony repair at injury sites. Bony repair is preceded by an injury-induced inflammatory response, which could play a role in regulating the repair process. Here, roles of two inflammatory mediators, cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS), in the injury responses were analysed by examining their gene expression and effects of blocking their activities, respectively, with celecoxib and aminoguanidine during 2 days prior to and until 7 days after injury in a rat tibial growth plate injury model. Quantitative RT-PCR assays revealed upregulated expression of COX-2 on days 1 and 4 and iNOS on day 1. Histological analysis of injury sites revealed significant reductions in inflammatory infiltrate (particularly neutrophils) on day 1 in treated groups compared to saline control. While bony tissue proportions at injury sites were unaffected by either treatment, mesenchymal tissue proportions were larger but cartilaginous tissue proportions were smaller on day 8 (though statistically insignificant), and bone remodelling appeared delayed with a smaller bone marrow proportion on day 14 in both treatment groups. These findings su...Continue Reading

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Citations

Jun 24, 2008·BMC Developmental Biology·Esther J PearlCaroline W Beck
Feb 5, 2010·The Journal of Clinical Endocrinology and Metabolism·Sander PouwelsFrank de Vries
Jul 4, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Fiona H ZhouCory J Xian
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Nov 13, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christopher B EricksonKarin A Payne

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