Roles of E4orf6 and VA I RNA in adenovirus-mediated stimulation of human parvovirus B19 DNA replication and structural gene expression.

Journal of Virology
Kerstin WinterStefan Weger

Abstract

Despite its very narrow tropism for erythroid progenitor cells, human parvovirus B19 (B19V) has recently been shown to replicate and form infectious progeny virus in 293 cells in the presence of early adenoviral functions provided either by infection with adenovirus type 5 or by addition of the pHelper plasmid encoding the E2a, E4orf6, and VA RNA functions. In the present study we dissected the individual influence of these functions on B19V genome replication and expression of structural proteins VP1 and VP2. We show that, in the presence of the constitutively expressed E1A and E1B, E4orf6 alone is able to promote B19V DNA replication, resulting in a concomitant increase in VP expression levels. The stimulatory effects of E4orf6 require the integrity of the BC box motifs, which target cellular proteins such as p53 and the Mre11 DNA repair complex for proteosomal degradation through formation of an E3 ubiquitin ligase complex with E1B. VA RNA also strongly induces VP expression but, in contrast to E4orf6, in a replication-independent manner. This stimulation could be attributed exclusively to the VA I RNA transcript and does not involve major activating effects at the level of the B19V p6 promoter, but the nucleotide residues r...Continue Reading

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Citations

Jan 19, 2016·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·A Servant-Delmas, F Morinet
Oct 15, 2014·Biomolecules·Cathal MahonElah Pick
Dec 24, 2014·The Journal of General Virology·Catrin StutikaRegine Heilbronn
Jul 21, 2015·Biochemical and Biophysical Research Communications·Natalja RutzStefan Weger
Jun 23, 2015·Future Virology·Yong Luo, Jianming Qiu
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Mar 30, 2019·The Journal of General Virology·Bertfried MatzAnna Maria Eis-Hübinger
Feb 18, 2016·Molecular Biology Reports·Azadeh AnbarlouGiorgio Gallinella
Mar 28, 2021·Virus Research·Ashish Prasad, Manoj Prasad
Aug 15, 2017·Virology·Tyler JanovitzErik Falck-Pedersen

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