Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin

Molecular Medicine Reports
Xia KongXiao-Qing Chen

Abstract

Previous studies investigating the role of toll-like receptors (TLRs) in asthma have been inconclusive. It has remained elusive whether the toll-like receptors (TLR2)/mycloid differentiation factor 88 (MyD88)/nuclear factor (NF)-κB signaling pathway is involved in lipoxin A4 (LXA4)-induced protection against asthma. Therefore, the present study investigated whether ovalbumin (OVA)-induced airway inflammation is mediated by upregulation of the TLR2/MyD88/NF-κB signaling pathway, and whether it proceeds via the inhibition of the activation of the LXA4 receptor and anti-interleukin (IL)-1β antibodies. Mice with airway inflammation induced by OVA administration were treated with or without a LXA4 receptor agonist, BML-111 and anti-IL-1β antibody. Serum levels of IL-1β, IL-4, IL-8 and interferon-γ (IFN-γ) were assessed, and levels of IL-1β, IL-4, IL-8 and OVA-immunoglobulin (Ig)E, as well as leukocyte counts in the bronchoalveolar lavage fluid (BALF) were measured. Pathological features and expression of TLR2, MyD88 and NF-κB in the lungs were analyzed. Expression of TLR2 and MyD88, and activation of NF-κB in leukocytes as well as levels of IL-4, IL-6 and IL-8 released from leukocytes exposed to IL-1β were assessed. OVA treatment in...Continue Reading

References

May 1, 1992·The Journal of Allergy and Clinical Immunology·D H BroideS I Wasserman
Jun 1, 1992·The American Review of Respiratory Disease·P E ChristieT H Lee
Nov 14, 1991·Biochemical and Biophysical Research Communications·T H LeeB W Spur
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·P M O'Byrne
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·A R Leff
Nov 3, 2001·The Journal of Allergy and Clinical Immunology·J Y ChoD H Broide
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew T GewirtzJames L Madara
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Caroline BonnansPascal Chanez
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I SabroeS K Dower
Aug 13, 2002·Nature Medicine·Bruce D LevyCharles N Serhan
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vanessa RedeckeEyal Raz
Sep 3, 2004·Nature Reviews. Immunology·Andrew D Luster, Andrew M Tager
Dec 4, 2004·American Journal of Respiratory Cell and Molecular Biology·German VelascoPatricia W Finn
Jan 22, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Xiaoxia Li, Jinzhong Qin
May 6, 2006·The Journal of Allergy and Clinical Immunology·Tsuneyasu Kaisho, Shizuo Akira
Jul 18, 2006·Thorax·Jodie L SimpsonPeter G Gibson
Mar 3, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Sheng-Hua WuLing Dong
Jul 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bruce D LevyJohn F Parkinson
Jul 24, 2008·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·L ZhangJ Wan
Sep 9, 2008·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Sheng-Hua WuZ-Q Chen
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Iris HechtYossi Cohen
Jan 6, 2010·EMBO Molecular Medicine·Stephanie C Eisenbarth, Richard A Flavell
May 29, 2012·The Journal of Allergy and Clinical Immunology·Deepti R NagarkarAtsushi Kato
Aug 14, 2012·Current Opinion in Allergy and Clinical Immunology·Karoline KrauseMarcus Maurer
Sep 22, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D S FerreiraT Mauad
Mar 16, 2013·American Journal of Respiratory Cell and Molecular Biology·Christopher BauerJill A Poole

❮ Previous
Next ❯

Citations

Mar 11, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ho Pan ShamBruce D Levy
Jun 4, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda CroasdellRichard P Phipps
Dec 31, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Ghada S El-TanboulyHatem A Salem
Jul 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Renata M MartinezRubia Casagrande

❮ Previous
Next ❯

Methods Mentioned

BETA
bronchoalveolar lavage
transgenic
electrophoretic mobility shift assay
ELISA
lavage
light microscopy
Protein Extraction
Assay
X-ray
FCS

Software Mentioned

Statistical Package for Social Sciences

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.