Roles of mammalian sterile 20-like kinase 2-dependent phosphorylations of Mps one binder 1B in the activation of nuclear Dbf2-related kinases

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Yijun BaoY Hata

Abstract

Mammalian nuclear Dbf2-related (NDR) kinases (LATS1, LATS2, NDR1 and NDR2) play a role in cell proliferation, apoptosis and morphological changes. Mammalian sterile 20-like (MST) kinases and Mps one binder (MOB) proteins are important in the activation of NDR kinases. MOB1 is phosphorylated by MST1 and MST2 and this phosphorylation enhances the ability of MOB1 to activate NDR kinases. The phosphorylated MOB1 can be more effective as a scaffold protein to facilitate the MST-dependent phosphorylation of NDR kinases and/or as a direct activator of NDR kinases. We previously reported that Thr74 of MOB1B is phosphorylated by MST2. Thr12 and Thr35 have also been identified as phosphorylation sites. In this study, we quantified the phosphorylation of Thr74 using the phosphorylated Thr74-specific antibody. Thr74 is indeed phosphorylated by MST2, but the efficiency is low, suggesting that MOB1B can activate NDR kinases without the phosphorylation of Thr74. We also showed that the phosphorylated MOB1B activates NDR1 T444D and LATS2 T1041D, in which threonine residues phosphorylated by MST kinases are replaced with phosphorylation-mimicking aspartic acid, more efficiently than the unphosphorylated MOB1B does. This finding supports that th...Continue Reading

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Citations

Aug 13, 2011·Molecular Biology of the Cell·Murugan ParamasivamDannel McCollum
Mar 16, 2013·Marine Drugs·Yutaka HataSainawaer Maimaiti
May 5, 2011·Cellular Signalling·Alexander Hergovich
Jun 12, 2012·Biochimica Et Biophysica Acta·Angela M LiuJohn M Luk
Jun 24, 2016·Scientific Reports·Sun-Yong KimToshio Hakoshima
Mar 5, 2016·PLoS Genetics·Xiaona CuiYoufa Cheng
Jul 28, 2016·Molecular and Cellular Biology·Radhika JagannathanGregory D Longmore
Oct 25, 2017·Molecular Cancer Research : MCR·Junichi MaruyamaYutaka Hata
Aug 25, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Momotaj JahanYutaka Hata

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