Roles of methyltrienolone (R1881) in AKTs and AR expression patterns of cultured granulosa-lutein cells

Journal of Cellular Biochemistry
Saeid NekoonamFardin Amidi

Abstract

AR-mediated androgen signaling plays a key role in female reproductive system. Granulosa-lutein cells (GCs) are the main sites for expression of androgen receptor (AR). There is also a close relation between AKT signaling and AR. Here, we assayed the role for a synthetic AR ligand methyltrienolone (R1881) in expressions of AKTs and AR. Controlled ovarian hyperstimulation (COH) was performed in 20 normal women. Mural GCs were isolated by filtration method, cultured, and passaged. Then, the cells were starved for 48 h with 10% charcoal stripped FBS. The cells were then treated with R1881, bicalutamide (AR blocker), LY294002 (PI3K/AKT pathway blocker), and combination of them for 48 h. Finally, GCs were evaluated for quantitative real-time PCR analysis of AKT1, AKT2, AKT3, and AR, and also Western blot assessment of total AKT and phosphorylated AKT (p-AKT) [Ser473 and Thr308]. Addition of R1881 to the GCs culture showed high expressions of AKT1, AKT2, and AKT3 (P ≤ 0.05 vs LY294002 group and bicalutamide group). Expressions of AKT1 and AKT2 were decreased in the GCs under exposure to bicalutamide or LY294002 (P ≤ 0.05 vs R1881). AKT1, AKT2, and AKT3 showed decreased rates of expressions in the LY294002 + bicalutamide group (P ≤ 0....Continue Reading

References

Sep 22, 1999·European Journal of Biochemistry·S MasureA Richardson
Jul 28, 2007·Human Reproduction·Saveria AquilaSebastiano Andò
Sep 5, 2008·Diabetes Care·Mark O GoodarziRicardo Azziz
Jul 30, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Debashis SarkerJohann S de Bono
Nov 28, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Barbara J AttardiAlvin M Matsumoto
Sep 16, 2011·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Annu MakkerAnjoo Agarwal
Mar 28, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Sylvain GauthierFernand Labrie
Feb 19, 2013·The International Journal of Developmental Biology·Sandra CecconiFelice Patacchiola
Mar 5, 2013·Endocrine-related Cancer·Rhonda L Bitting, Andrew J Armstrong
Sep 13, 2013·Molecular Human Reproduction·M Lebbe, T K Woodruff
Oct 18, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·JongYeob ChoiDooSeok Choi
Oct 15, 2014·Journal of Molecular Endocrinology·Annu MakkerAbbas Ali Mahdi
Nov 18, 2015·Canadian Journal of Physiology and Pharmacology·Keywan MortezaeeIraj Ragerdi Kashani
Jan 16, 2016·Tissue & Cell·Luiz Gustavo de Almeida ChuffaAlfredo Feio da Maia Lima
Oct 30, 2016·Molecular and Cellular Endocrinology·Shifeng SuElizabeth M Wilson
Mar 9, 2017·Archives of Gynecology and Obstetrics·Saeid NekoonamFardin Amidi
Oct 13, 2017·Journal of Cellular Physiology·Keywan Mortezaee, Neda Khanlarkhani
Oct 27, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·H T NieF Wang
Nov 19, 2017·Journal of Cellular Physiology·Keywan MortezaeeAdib Zendedel
Jan 5, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Chong FengXuan Zhang

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