Roles of minor pilin subunits Spy0125 and Spy0130 in the serotype M1 Streptococcus pyogenes strain SF370.

Journal of Bacteriology
Wendy D SmithMichael A Kehoe

Abstract

Adhesive pili on the surface of the serotype M1 Streptococcus pyogenes strain SF370 are composed of a major backbone subunit (Spy0128) and two minor subunits (Spy0125 and Spy0130), joined covalently by a pilin polymerase (Spy0129). Previous studies using recombinant proteins showed that both minor subunits bind to human pharyngeal (Detroit) cells (A. G. Manetti et al., Mol. Microbiol. 64:968-983, 2007), suggesting both may act as pilus-presented adhesins. While confirming these binding properties, studies described here indicate that Spy0125 is the pilus-presented adhesin and that Spy0130 has a distinct role as a wall linker. Pili were localized predominantly to cell wall fractions of the wild-type S. pyogenes parent strain and a spy0125 deletion mutant. In contrast, they were found almost exclusively in culture supernatants in both spy0130 and srtA deletion mutants, indicating that the housekeeping sortase (SrtA) attaches pili to the cell wall by using Spy0130 as a linker protein. Adhesion assays with antisera specific for individual subunits showed that only anti-rSpy0125 serum inhibited adhesion of wild-type S. pyogenes to human keratinocytes and tonsil epithelium to a significant extent. Spy0125 was localized to the tip of ...Continue Reading

References

Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H Ton-ThatO Schneewind
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·James C SmootJames M Musser
Nov 19, 2003·Molecular Microbiology·Hung Ton-That, Olaf Schneewind
Jul 1, 2004·Molecular Microbiology·Hung Ton-ThatOlaf Schneewind
Jul 5, 2005·Science·Peter LauerJohn L Telford
Oct 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marirosa MoraJohn L Telford
Feb 3, 2006·Journal of Bacteriology·Andrew H Gaspar, Hung Ton-That
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·M A BarocchiB Henriques-Normark
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Luciano A MarraffiniOlaf Schneewind
Jun 7, 2006·Annual Review of Microbiology·June R Scott, Timothy C Barnett
Jun 17, 2006·Nature Reviews. Microbiology·John L TelfordGuido Grandi
Jun 27, 2006·Molecular Microbiology·Shaynoor DramsiPatrick Trieu-Cuot
Sep 19, 2006·Molecular Microbiology·June R Scott, Dorothea Zähner
Oct 3, 2006·Journal of Bacteriology·Matthew T G HoldenJulian Parkhill
Oct 10, 2006·Journal of Bacteriology·Zerina KratovacDebra E Bessen
Mar 16, 2007·Cellular Microbiology·Emily L AbbotMichael A Kehoe
May 16, 2007·Molecular Microbiology·Andrea G O ManettiImmaculada Margarit
Sep 14, 2007·Molecular Microbiology·A L NelsonB Henriques-Normark
Sep 28, 2007·Molecular Microbiology·Jonathan M BudzikOlaf Schneewind
Dec 18, 2007·Trends in Microbiology·Anjali MandlikHung Ton-That
Feb 13, 2008·FEMS Microbiology Reviews·Shaynoor DramsiMichel Arthur
Jul 9, 2008·Journal of Bacteriology·Julianna LeMieuxAndrew Camilli
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anjali MandlikHung Ton-That
Oct 23, 2008·The Journal of Biological Chemistry·Jonathan M BudzikOlaf Schneewind
Mar 18, 2009·European Biophysics Journal : EBJ·Alexandra S SolovyovaMark J Banfield
May 13, 2009·Molecular Microbiology·Bernard R QuigleyJune R Scott
Jul 14, 2009·Journal of Bacteriology·Irene K GuttillaHung Ton-That
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hae Joo KangEdward N Baker
Nov 28, 2009·The EMBO Journal·Markus HilleringmannAndreas Engel

❮ Previous
Next ❯

Citations

Aug 24, 2010·The Journal of Biological Chemistry·Jonathan A PointonMark J Banfield
May 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Vengadesan KrishnanSthanam V L Narayana
Oct 4, 2011·PloS One·Dorothea ZähnerDavid S Stephens
Feb 22, 2012·Current Opinion in Structural Biology·Hae Joo Kang, Edward N Baker
Oct 27, 2011·Molecular Microbiology·Thomas SpirigRobert T Clubb
Jun 12, 2013·Journal of Structural Biology·Paul G YoungEdward N Baker
Jun 18, 2016·FEBS Letters·Stephan BrouwerMark J Walker
Nov 30, 2010·International Journal of Medical Microbiology : IJMM·Bernd KreikemeyerAndreas Podbielski
Mar 30, 2017·The Journal of Biological Chemistry·Daniel J EchelmanJulio M Fernández
Apr 4, 2014·Clinical Microbiology Reviews·Mark J WalkerVictor Nizet
Feb 18, 2011·Nature Reviews. Microbiology·Antoni P A HendrickxOlaf Schneewind
Apr 6, 2019·Current Drug Targets·Heema K N VyasMartina Sanderson-Smith
Mar 19, 2011·Current Opinion in Infectious Diseases·Nicola N LynskeyShiranee Sriskandan
Nov 14, 2013·The Journal of Biological Chemistry·Christian Linke-WinnebeckEdward N Baker
May 3, 2018·Pathogens and Disease·J M RaynesN J Moreland
Feb 27, 2021·Frontiers in Microbiology·Masanobu Nakata, Bernd Kreikemeyer
Mar 2, 2021·Annual Review of Biophysics·Souradeep BanerjeeShubhasis Haldar
May 22, 2019·Microbiology Spectrum·Claire E TurnerAndroulla Efstratiou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.