Roles of nifF and nifJ gene products in electron transport to nitrogenase in Klebsiella pneumoniae.

Journal of Bacteriology
S Hill, E P Kavanagh

Abstract

Crude extracts of the wild-type Klebsiella pneumoniae reduced C2H2 with either pyruvate or formate as reductant (specific activity, 3 nmol min-1 mg of protein-1), whereas crude extracts of nifF mutant were almost inactive (specific activity, 0.05). However, activity in the latter extracts was stimulated by adding Azotobacter chroococcum flavodoxin (specific activity, 10). Thus, nifF mutants may lack an electron transport factor. Crude extracts of nifJ mutants had about 20% of the wild-type level of active MoFe protein, and thus nifJ has a presumptive role in maintaining active MoFe protein. Studies on pyruvate or formate as reductants for nitrogenase in extracts of the nifJ mutants suggest in addition a role in electron input to nitrogenase for the following reasons. (i) Nitrogenase activity with these reductants was very low (specific activity, 0.06) and was not stimulated by extra MoFe protein or the flavodoxin. (ii) Activity was increased by adding a crude extract of a mutant lacking the structural nif genes (specific activity, 1) or a crude extract of the nifF mutant (specific activity, 4).

References

Jan 1, 1976·Advances in Microbial Physiology·J A Cole
Nov 29, 1977·Molecular & General Genetics : MGG·R DixonM Merrick
Dec 1, 1976·Journal of General Microbiology·P R Lambden, J R Guest
Mar 1, 1975·Journal of Bacteriology·R T St JohnW J Brill
Aug 10, 1966·Biochimica Et Biophysica Acta·H G Hansen, U Henning
Jan 1, 1974·Journal of General Microbiology·F C CannonS B Primrose
Mar 1, 1971·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H Vetter, J Knappe
Aug 1, 1965·The Biochemical Journal·I R HamiltonP W Wilson
Aug 31, 1970·FEBS Letters·R K. ThauerK Jungermann
Jun 1, 1965·Journal of Bacteriology·M C MAHLW H EWING

❮ Previous
Next ❯

Citations

Aug 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianguo YangYi-Ping Wang
Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Feb 15, 2011·Applied and Environmental Microbiology·Kelsey McNeelyG Charles Dismukes
Apr 21, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Yang Liu, Baojun Wang
Jan 11, 2013·International Journal of Molecular Sciences·Inmaculada Pérez-DoradoJuan A Hermoso
Feb 22, 2016·Microbial Cell Factories·Xin-Xin LiSan-Feng Chen
Apr 24, 2016·FEMS Microbiology Ecology·Mauro Degli Esposti, Esperanza Martinez Romero
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nan XiangYi-Ping Wang
Mar 22, 2020·Applied and Environmental Microbiology·Haowen ShiSanfeng Chen
Feb 28, 2018·Journal of Bacteriology·Saroj PoudelEric S Boyd
Jan 31, 2020·Chemical Reviews·Hannah L Rutledge, F Akif Tezcan
Apr 1, 1982·Journal of Bacteriology·G StaceyW J Brill
Jul 1, 1996·Journal of Bacteriology·G GennaroP C Hallenbeck
Apr 1, 1990·Journal of Bacteriology·S HillC Anthony
Dec 1, 1988·Applied and Environmental Microbiology·R M LankhorstR C van den Bos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.