PMID: 8445717Apr 1, 1993Paper

Roles of nonstructural polyproteins and cleavage products in regulating Sindbis virus RNA replication and transcription

Journal of Virology
J A Lemm, C M Rice

Abstract

Using vaccinia virus to express Sindbis virus (SIN) nonstructural proteins (nsPs) and template RNAs, we showed previously that synthesis of all three viral RNAs occurred only during expression of either the entire nonstructural coding region or the polyprotein precursors P123 and P34. In this report, the vaccinia virus system was used to express cleavage-defective polyproteins and nsP4 proteins containing various N-terminal extensions to directly examine the roles of the P123 and P34 polyproteins in RNA replication. Replication and subgenomic mRNA transcription occurred during coexpression of P34 and P123 polyproteins in which cleavage was blocked at either or both of the 1/2 and 2/3 sites. For all cleavage-defective P123 polyproteins, however, the ratio of subgenomic to genomic RNA was decreased, suggesting that both the 1/2 and 2/3 cleavages are required for efficient subgenomic RNA transcription. These studies indicate that the uncleaved P123 polyprotein can function as a component of the viral replicase capable of synthesizing both plus- and minus-strand RNAs. In contrast, cleavage-defective P34 was unable to function in RNA replication, even in complementation experiments in which minus-strand RNAs were provided by nsP4. A...Continue Reading

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Citations

Aug 15, 2003·The Journal of Biological Chemistry·Lidia VasiljevaTero Ahola
Aug 8, 2008·Journal of Virology·Rodion GorchakovIlya Frolov
Dec 18, 2009·Journal of Virology·Margus VarjakAndres Merits
Sep 10, 2010·Journal of Virology·Elena I FrolovaIlya Frolov
Jul 3, 2013·Annual Review of Microbiology·Brian R Wasik, Paul E Turner
Aug 20, 2015·Viruses·Colleen R ReidTom C Hobman
Jun 18, 2020·Veterinary Research·Georges SaadeFrançois Meurens
Jan 21, 2011·Journal of Virology·Jonathan C RuppRichard W Hardy
Dec 3, 2003·Journal of Virology·Indra DéDorothea L Sawicki
Mar 31, 2006·Journal of Virology·Elena FrolovaIlya Frolov
Aug 31, 1999·The Journal of General Virology·E ten DamM D Ryan
Aug 7, 2002·Journal of Virology·Cori L FataDorothea L Sawicki
Apr 18, 2008·Journal of Virology·Rodion GorchakovIlya Frolov
Jul 11, 1998·Journal of Virology·J A LemmC M Rice
Mar 11, 2006·The Journal of General Virology·Sareen E GalbraithGregory J Atkins

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