Roles of outer membrane protein W (OmpW) on survival, morphology, and biofilm formation under NaCl stresses in Cronobacter sakazakii

Journal of Dairy Science
Yingwang YeQingping Wu

Abstract

Cronobacter sakazakii is an important foodborne pathogen associated with rare but severe infections through consumption of powdered infant formula. Tolerance to osmotic stress in Cronobacter has been described. However, the detailed factors involved in tolerance to osmotic stress in C. sakazakii are poorly understood. In this study, roles of outer membrane protein W (OmpW) on survival rates, morphologic changes of cells, and biofilm formation in C. sakazakii under different NaCl concentrations between wild type (WT) and OmpW mutant (ΔOmpW) were determined. The survival rates of ΔOmpW in Luria-Bertani medium with 3.5% or 5.5% NaCl were reduced significantly, and morphological injury of ΔOmpW was significantly increased compared with survival and morphology of WT. Compared with biofilm formation of the WT strain, biofilms in ΔOmpW were significantly increased in Luria-Bertani with 3.5% or 5.5% NaCl using crystal violet staining assay after 48 and 72 h of incubation. Detection of biofilms using confocal laser scanning microscopy and scanning electron microscopy further confirmed the changes of biofilm formation under different NaCl stresses. This study demonstrates that OmpW contributes to survival of cells in planktonic mode unde...Continue Reading

References

Sep 1, 1989·Journal of Clinical Microbiology·G BieringO Steingrímsson
Jul 1, 1983·Journal of Clinical Microbiology·H L MuytjensJ J Farmer
Nov 25, 2003·Journal of Applied Microbiology·P BreeuwerH M Joosten
Nov 30, 2004·Research in Microbiology·Changxin XuXuanxian Peng
Jul 26, 2005·International Journal of Food Microbiology·Joshua B GurtlerLarry R Beuchat
Sep 2, 2006·Journal of Proteome Research·Lina WuXuanxian Peng
Dec 5, 2009·Foodborne Pathogens and Disease·Brendan HealySéamus Fanning
Feb 4, 2010·The Journal of Microbiology·Ji-Hyun ShinJungmin Kim
Jun 15, 2010·Applied and Environmental Microbiology·Kyumson KimSangryeol Ryu
Jul 13, 2012·Journal of Clinical Microbiology·S JosephS J Forsythe
Jan 21, 2014·Foodborne Pathogens and Disease·Xiaoke XuXiaohui Dong

❮ Previous
Next ❯

Citations

Jul 25, 2019·Frontiers in Microbiology·Mohamed A AlyErik Reimhult
Sep 25, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Muzamil Ahmad RatherManabendra Mandal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.