Roles of phosphatidylinositol 3-kinase in root hair growth.

Plant Physiology
Yuree LeeYoungsook Lee

Abstract

The root hair is a model system for understanding plant cell tip growth. As phosphatidylinositol 3-phosphate [PtdIns(3)P] has been shown in other plant cell types to regulate factors that affect root hair growth, including reactive oxygen species (ROS) levels, cytoskeleton, and endosomal movement, we hypothesized that PtdIns(3)P is also important for root hair elongation. The enzyme that generates PtdIns(3)P, phosphatidylinositol 3-kinase (PI3K), was expressed in root hair cells of transgenic plants containing the PI3K promoter:beta-glucuronidase reporter construct. To obtain genetic evidence for the role of PtdIns(3)P in root hair elongation, we attempted to isolate Arabidopsis (Arabidopsis thaliana) mutant plants that did not express the gene VPS34 encoding the PI3K enzyme. However, the homozygous mutant was lethal due to gametophytic defects, and heterozygous plants were not discernibly different from wild-type plants. Alternatively, we made transgenic plants expressing the PtdIns(3)P-binding FYVE domain in the root hair cell to block signal transduction downstream of PtdIns(3)P. These transgenic plants had shorter root hairs and a reduced hair growth rate compared with wild-type plants. In addition, LY294002, a PI3K-specifi...Continue Reading

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Citations

Jan 22, 2010·Protoplasma·Till IschebeckIngo Heilmann
Apr 25, 2012·Journal of Proteome Research·Tomáš TakáčJozef Samaj
Apr 10, 2010·Journal of Experimental Botany·Laura Zonia
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Feb 10, 2016·Frontiers in Plant Science·Daniel von WangenheimJozef Šamaj
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Feb 27, 2014·Molecular Plant·Jiameng ZhengMarcela Rojas-Pierce

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