PMID: 7539396Jun 1, 1995Paper

Roles of prostaglandin production and mitogen-activated protein kinase activation in hepatocyte growth factor-mediated rat hepatocyte proliferation

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
T AdachiY Nozawa

Abstract

Hepatocyte growth factor (HGF) and epidermal growth factor (EGF)-stimulated DNA synthesis in primary cultured rat hepatocytes. HGF-induced DNA synthesis was concentration-dependently inhibited by a cyclooxygenase inhibitor, indomethacin. BW755C, a dual inhibitor for cyclooxygenase and lipoxygenase activities, also inhibited hepatocyte growth. Prostaglandin E1 (PGE1), PGE2, and PGF2 alpha induced DNA synthesis even at such a low concentration as 5 nmol/L and potentiated [3H]thymidine incorporation induced by HGF in an additive manner. HGF caused arachidonic acid (AA) release and eicosanoid production. These events were detectable within 10 minutes after stimulation and lasted for at least 60 minutes. Furthermore, two proteins with approximately 40 kd were tyrosine phosphorylated by HGF. These proteins were identified as p42/p44 mitogen-activated protein (MAP) kinases by anti-MAP kinase immunoblots, which were known to activate cytosolic phospholipase A2 (cPLA2), a key enzyme in AA release. Activation of MAP kinases was detectable within 5 minutes after stimulation with HGF and lasted for at least 60 minutes. EGF-mediated DNA synthesis was also inhibited by the above cyclooxygenase inhibitors. EGF caused AA release and tyrosine p...Continue Reading

References

Jan 15, 1979·Analytical Biochemistry·C F BrunkT W James
Jun 30, 1992·Biochemical and Biophysical Research Communications·Y ChataniM Kohno
Jan 31, 1991·Biochemical and Biophysical Research Communications·T IgawaT Nakamura
Aug 15, 1991·Biochemical and Biophysical Research Communications·G G Skouteris, M R Kaser
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S RubinP W Finch
Apr 30, 1991·Biochemical and Biophysical Research Communications·O Higuchi, T Nakamura
Dec 31, 1991·Biochemical and Biophysical Research Communications·T MineT Nakamura
Sep 1, 1991·Experimental Cell Research·K MatsumotoT Nakamura
Jul 1, 1990·Journal of Neurochemistry·T Shimizu, L S Wolfe
Sep 15, 1989·Biochemical and Biophysical Research Communications·K MiyazawaK Takahashi
Jun 1, 1988·Journal of Cellular Physiology·G G SkouterisL A Stocken
Aug 16, 1984·Biochemical and Biophysical Research Communications·T NakamuraA Ichihara
Jan 29, 1993·Cell·L L LinR J Davis
Apr 1, 1993·Trends in Biochemical Sciences·E Nishida, Y Gotoh

❮ Previous
Next ❯

Citations

Oct 23, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B Della GasperaS Weinman
Jan 1, 1997·Prostaglandins·R Flisiak, D Prokopowicz
May 5, 1999·Critical Reviews in Oncology/hematology·W JiangT Nakamura
Dec 1, 2000·Biochemical and Biophysical Research Communications·T NomiH Kawasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.