Roles of siderophores, oxalate, and ascorbate in mobilization of iron from hematite by the aerobic bacterium Pseudomonas mendocina.

Applied and Environmental Microbiology
Carolyn A DehnerJennifer L Dubois

Abstract

In aerobic, circumneutral environments, the essential element Fe occurs primarily in scarcely soluble mineral forms. We examined the independent and combined effects of a siderophore, a reductant (ascorbate), and a low-molecular-weight carboxylic acid (oxalate) on acquisition of Fe from the mineral hematite (alpha-Fe(2)O(3)) by the obligate aerobe Pseudomonas mendocina ymp. A site-directed DeltapmhA mutant that was not capable of producing functional siderophores (i.e., siderophore(-) phenotype) did not grow on hematite as the only Fe source. The concentration of an added exogenous siderophore (1 microM desferrioxamine B [DFO-B]) needed to restore wild-type (WT)-like growth kinetics to the siderophore(-) strain was approximately 50-fold less than the concentration of the siderophore secreted by the WT organism grown under the same conditions. The roles of a reductant (ascorbate) and a simple carboxylic acid (oxalate) in the Fe acquisition process were examined in the presence and absence of the siderophore. Addition of ascorbate (50 microM) alone restored the growth of the siderophore(-) culture to the WT levels. A higher concentration of oxalate (100 microM) had little effect on the growth of a siderophore(-) culture; however,...Continue Reading

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Citations

Feb 19, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Carolyn DehnerJennifer L Dubois
May 12, 2011·Environmental Science & Technology·Juraj Majzlan
Nov 6, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Ana Luiza Costa MattosBreno Pannia Espósito
Apr 12, 2016·Frontiers in Microbiology·Damien ParrelloPatrick Billard
Jan 27, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·James M HarringtonKurt Haselwandter
May 31, 2015·Applied and Environmental Microbiology·Mahmoud Kamal AhmadiBlaine A Pfeifer
Apr 30, 2017·Environmental Science and Pollution Research International·Qingqi LinRongliang Qiu
Dec 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Caroll M MendoncaLudmilla Aristilde
May 13, 2015·Environmental Science & Technology·Zimeng WangDaniel E Giammar
Dec 23, 2010·Environmental Science & Technology·Carolyn A DehnerJennifer L DuBois
Oct 20, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Caylyn A LanzlDavid M Cwiertny

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