Roles of some antioxidants in modulation of cardiac myopathy induced by sodium nitrite via down-regulation of mRNA expression of NF-κB, Bax, and flt-1 and suppressing DNA damage

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
Laila Mohamed FaddaNawal Mohamed Al-Rasheed

Abstract

The underlying pathology of cardiac damage involves various molecular and signaling pathways. Therefore, this study aimed to explore the role of Quercetin (Querc), alone or in combination with Melatonin (Melat) against cardiac damage induced by sodium nitrite (Sod nit), as well as to elucidate different signaling pathways. Querc and Melat were injected intraperitoneally (i.p.), followed by induction of hypoxia in rats by using a single dose of Sod nit (60 mg/kg, s.c.). Treatment with Sod nit significantly decreased hemoglobin (Hb) levels in blood. Pretreatment of hypoxic rats with Querc and/or Melat elevated the declined Hb concentration. The forementioned antioxidants also successfully ameliorated the alteration of heat shock protein 70 (HSP-70) and markers of cardiac injury, including troponin T (Trop. T), creatine kinase-MB (CK-MB), tumor necrosis factor-α (TNF α), and C-reactive protein (CRP) in the rats serum. Furthermore, RT-PCR revealed that these antioxidants successfully modulated mRNA expression of NF-κB, Bax, Bcl-2, and flt-1. They also regulated vascular endothelial growth factor (VEGF), the apoptosis marker caspase 3, and oxidative DNA damage in cardiac tissue, compared to Sod nit-intoxicated rats. The present bioc...Continue Reading

Citations

Dec 5, 2019·Dose-response : a Publication of International Hormesis Society·Ahlam M AlhusainiAbeer Al-Anazi
Mar 1, 2020·International Journal of Molecular Sciences·Kristina FerenczyovaMonika Bartekova
Feb 10, 2020·Environmental Science and Pollution Research International·Nagla A El-NabarawyLaila A Rashed
Nov 6, 2020·Environmental Science and Pollution Research International·Mai M EissaSahar H Orabi

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Methods Mentioned

BETA
ELISA
electrophoresis
PCR

Software Mentioned

Sod

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