Roles of Soybean Plasma Membrane Intrinsic Protein GmPIP2;9 in Drought Tolerance and Seed Development

Frontiers in Plant Science
Linghong LuHuixia Shou

Abstract

Aquaporins play an essential role in water uptake and transport in vascular plants. The soybean genome contains a total of 22 plasma membrane intrinsic protein (PIP) genes. To identify candidate PIPs important for soybean yield and stress tolerance, we studied the transcript levels of all 22 soybean PIPs. We found that a GmPIP2 subfamily member, GmPIP2;9, was predominately expressed in roots and developing seeds. Here, we show that GmPIP2;9 localized to the plasma membrane and had high water channel activity when expressed in Xenopus oocytes. Using transgenic soybean plants expressing a native GmPIP2;9 promoter driving a GUS-reporter gene, it was found high GUS expression in the roots, in particular, in the endoderm, pericycle, and vascular tissues of the roots of transgenic plants. In addition, GmPIP2;9 was also highly expressed in developing pods. GmPIP2;9 expression significantly increased in short term of polyethylene glycol (PEG)-mediated drought stress treatment. GmPIP2;9 overexpression increased tolerance to drought stress in both solution cultures and soil plots. Drought stress in combination with GmPIP2;9 overexpression increased net CO2 assimilation of photosynthesis, stomata conductance, and transpiration rate, sugge...Continue Reading

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Citations

May 17, 2019·International Journal of Molecular Sciences·Malika AyadiKhaled Masmoudi
Dec 17, 2020·International Journal of Molecular Sciences·Yan WangFushun Hao
Dec 22, 2020·International Journal of Genomics·Aref AlshameriAbdalrhaman M Salih
Feb 23, 2020·Plant Physiology and Biochemistry : PPB·Roshan Kumar SinghManoj Prasad
Jul 1, 2020·Trends in Plant Science·Wei GuoLam-Son Phan Tran

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Methods Mentioned

BETA
transgenic
PCR
Assay
Fluorescence

Software Mentioned

Data Processing System ( DPS

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