PMID: 9554944May 12, 1998Paper

Roles of the bradyrhizobium japonicum terminal oxidase complexes in microaerobic H2-dependent growth

Biochimica Et Biophysica Acta
M A Surpin, R J Maier

Abstract

Spectral, inhibitor, and O2-consumption studies on membranes from free-living and bacteroid forms of Bradyrhizobium japonicum have revealed the existence of a number of terminal oxidases, and four terminal oxidase gene clusters within the heme-copper cytochrome family have been cloned. Here the complexes encoded by coxMNOP and coxWXYZ, genes with homology to CuA-containing cytochrome c oxidases and b-type ubiquinol oxidases respectively, are studied by analysis of mutants in each of the two oxidases and a double mutant in both of the terminal oxidase genes. Membranes from microaerobically incubated strain JHK12 (which contains an insertion in coxWXYZ) were deficient in levels of CO-reactive heme b, and both strains JHK12 and Bj3430 (the latter lacks coxMNOP) were deficient in CN--reactive cytochrome b. Membranes of the double mutant (strain JHKS4) retained less than 7% of the cytochrome b3 and 25% of the total CN--reactive cytochrome b of the wild type. Cyanide inhibition curves of oxygen uptake by wild-type membranes were triphasic, and only the phases inhibited by the highest (at about 50 &mgr;M CN-, attributed to cytochrome aa3) and the lowest (at approximately 0.1 &mgr;M) CN- were identifiable in the membranes from the two ...Continue Reading

References

Jan 1, 1992·Archives of Microbiology·M BottH Hennecke
Jun 1, 1990·Applied and Environmental Microbiology·R J MaierW Sray
May 30, 1989·Biochimica Et Biophysica Acta·M R O'Brian, R J Maier
Feb 1, 1985·Journal of Bacteriology·M R O'Brian, R J Maier
Jan 1, 1994·Antonie van Leeuwenhoek·J Batut, P Boistard
Sep 1, 1994·Journal of Bacteriology·J A García-HorsmanR B Gennis
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O PreisigH Hennecke

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Citations

May 16, 2014·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Karamjit SinghTimothy G Townsend
Aug 12, 2008·Journal of Bacteriology·William L FranckGary Stacey
Jan 5, 1999·Applied and Environmental Microbiology·M A Surpin, R J Maier

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