Roles of the C-terminal end of SecY in protein translocation and viability of Escherichia coli.

Journal of Bacteriology
Kazuhiko ChibaK Ito

Abstract

SecY, a central component of the membrane-embedded sector of protein translocase, contains six cytosolic domains. Here, we examined the importance of the C-terminal cytosolic region of SecY by systematically shortening the C-terminal end and examining the functional consequences of these mutations in vivo and in vitro. It was indicated that the C-terminal five residues are dispensable without any appreciable functional defects in SecY. Mutants missing the C-terminal six to seven residues were partially compromised, especially at low temperature or in the absence of SecG. In vitro analyses indicated that the initial phase of the translocation reaction, in which the signal sequence region of the preprotein is inserted into the membrane, was affected by the lack of the C-terminal residues. SecA binding was normal, but SecA insertion in response to ATP and a preprotein was impaired. It is suggested that the C-terminal SecY residues are required for SecA-dependent translocation initiation.

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Citations

Apr 19, 2011·Nature Structural & Molecular Biology·Jens FrauenfeldRoland Beckmann
Feb 15, 2011·The Journal of Biological Chemistry·Sanchaita Das, Donald B Oliver
Nov 18, 2010·Molecular Biology and Evolution·Janette TongTrevor Lithgow
Mar 25, 2004·Infection and Immunity·Nayoung KimGeorge Sachs
Dec 18, 2012·Biochemistry. Biokhimii︠a︡·T F Solov'evaO Yu Portnyagina
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki Mori, Koreaki Ito
Sep 3, 2008·Molecular Microbiology·Spyridoula KaramanouAnastassios Economou
Oct 3, 2006·Biochimica Et Biophysica Acta·Sharyn L Rusch, Debra A Kendall
Nov 17, 2009·Structure·James GumbartKlaus Schulten
Sep 11, 2002·FEBS Letters·Eli O van der SluisArnold J M Driessen
Aug 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangIshita Mukerji
May 21, 2003·Molecular Genetics and Genomics : MGG·N ShimokawaK Ito
Jan 11, 2018·The Journal of Biological Chemistry·Hiroyuki MoriYoshinori Akiyama

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