Roles of the highly conserved amino acids in the globular head and stalk region of the Newcastle disease virus HN protein in the membrane fusion process

Bioscience Trends
Cheng-Xi SunZhiyu Wang

Abstract

Newcastle disease virus (NDV), an avain paramyxovirus, has been assigned to the genus Avulavirus within the family Paramyxoviridae. It causes Newcastle disease (ND) that is a highly contagious and fatal viral disease affecting poultry and most species of birds. The hemagglutinin-neuraminidase (HN) protein of NDV has multiple functions including mediating hemadsorption (HAD), neuraminidase (NA), and fusion promotion activities affecting the process of viral attachment, entry, replication and dissemination. Fusion ability of the NDV was highly correlated to its virulence. Mutations in the HN globular head and headless HN of NDV were constructed to determinate the impact of highly conserved amino acids in the globular head of paramyxovirus HN proteins and the roles of the stalk region of HN in the fusion process. It was found that the interaction between F and HN mutants E401A, G402A, G468A, V469A, Y526A, and T527A was equal to that in F and wt HN. The mutations of G402A, G468A, V469A, and T527A had various effects on cell fusion promotion, receptor binding ability, and NA activity, but the membrane merging rate was comparable to wt HN. The elimination of hemadsorption ability and NA activity of E401A and Y526A resulted in the los...Continue Reading

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Sep 19, 1997·Trends in Microbiology·G B Melikyan, L V Chernomordik
Apr 13, 1999·Molecular Cell·K A BakerT S Jardetzky
Apr 12, 2002·Trends in Biochemical Sciences·Sergio G Peisajovich, Yechiel Shai
Apr 23, 2002·Journal of Virology·Richard K PlemperRoberto Cattaneo
Jul 23, 2003·Biochimica Et Biophysica Acta·Trudy G Morrison
Jul 23, 2003·Biochimica Et Biophysica Acta·Sergio G Peisajovich, Yechiel Shai
Sep 27, 2003·Journal of Virology·Kathryn A Gravel, Trudy G Morrison
Jan 20, 2004·Journal of Molecular Biology·Michael C LawrencePeter M Colman
Apr 28, 2004·Bioelectrochemistry·Yu A Chizmadzhev
Dec 21, 2005·Virology·Robert A LambTheodore S Jardetzky
May 9, 2006·Trends in Microbiology·Charles J Russell, Laura E Luque
Feb 27, 2007·FEBS Letters·Winfried WeissenhornYves Gaudin
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Jun 30, 2010·PLoS Pathogens·Rebecca Ellis Dutch
Feb 25, 2011·Expert Reviews in Molecular Medicine·Hector C Aguilar, Benhur Lee
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ping YuanTheodore S Jardetzky

❮ Previous
Next ❯

Citations

Mar 13, 2021·The Journal of General Virology·Ulrike BreitingerHans-Georg Breitinger

❮ Previous
Next ❯