Roles of Toll-like receptors 2 and 6 in the inflammatory response to Mycoplasma gallisepticum infection in DF-1 cells and in chicken embryos

Developmental and Comparative Immunology
Wei TianXiuli Peng

Abstract

While Mycoplasma gallisepticum (MG) is a major pathogen that causes chronic respiratory diseases in chicken, the molecular mechanism of MG infection is not clear. In this study, we investigated the roles of Toll-like receptor 2 (TLR2) and 6 (TLR6) in MG infection. We found that TLR2 type 2 (TLR2-2) and TLR6 had differential expressions in chicken embryo fibroblasts (DF-1 cells), where TLR6 was highly expressed, but TLR2-2 was barely expressed. Upon MG infection, TLR6 expression was upregulated, followed by upregulation of downstream factors, MyD88, NF-κB, IL2, IL6, and TNF-α. Knockdown of TLR6 expression by shRNA abolished the MG-induced inflammatory responses. More interestingly, in the presence of TLR6, TLR2-2 didn't respond to MG infection in DF-1 cells. When TLR6 was knocked down by shRNA, however, TLR2 was upregulated upon MG infection, which was followed by upregulation of proinflammatory genes. Finally, we tested effects of the MG infection on expression of TLR2-2 and TLR6 in the lungs and trachea tissues of chicken embryos. We found both TLR2-2 and TLR6 were upregulated upon MG infection, followed by upregulation of the downstream NF-κB-mediated inflammatory responses. This study was the first to report the differential...Continue Reading

References

Feb 27, 1999·Current Opinion in Immunology·E B Kopp, R Medzhitov
Jun 17, 2000·Infection and Immunity·F WinnerC Citti
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A OzinskyA Aderem
Jun 30, 2001·International Immunology·O TakeuchiS Akira
Nov 15, 2002·European Journal of Immunology·Michael MorrPeter F Mühlradt
Dec 24, 2002·Current Opinion in Immunology·Shizuo Akira
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Dec 4, 2004·Immunogenetics·Ahmet YilmazJames J Zhu
Dec 8, 2004·European Journal of Immunology·Ute Buwitt-BeckmannArtur J Ulmer
Jan 22, 2005·Veterinary Immunology and Immunopathology·Muhammad IqbalAdrian L Smith
Jul 7, 2007·Developmental and Comparative Immunology·Megumi HiguchiTsukasa Seya
Aug 30, 2008·FEMS Microbiology Reviews·Thomas Prescott AtkinsonKen B Waites
Feb 10, 2009·Veterinary Microbiology·Meghan May, Daniel R Brown
Mar 17, 2010·Journal of Veterinary Emergency and Critical Care·Jane E Sykes
Jun 8, 2010·BMC Veterinary Research·Myriam ChazelFrançois Poumarat
Oct 12, 2010·The Veterinary Clinics of North America. Small Animal Practice·Jane E Sykes
Feb 3, 2012·Cold Spring Harbor Perspectives in Biology·Kim Newton, Vishva M Dixit
Aug 1, 2012·Avian Pathology : Journal of the W.V.P.A·Laszlo StipkovitsBela Denes
Jan 12, 2013·Veterinary Immunology and Immunopathology·Michael St PaulShayan Sharif
Sep 10, 2013·Acta Veterinaria Scandinavica·Ursula FürnkranzMichael P Szostak

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Citations

Apr 26, 2020·Pathogens·Sergei N BorchseniusNikolai A Barlev
Feb 3, 2021·Antioxidants·Peter F SuraiMichael T Kidd

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