Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC.

The Journal of Biological Chemistry
Fumio HayashiMasahiro Ishiura

Abstract

Cyanobacterial clock protein KaiC has a hexagonal, pot-shaped structure composed of six identical dumbbell-shaped subunits. Each subunit has duplicated domains, and each domain has a set of ATPase motifs. The two spherical regions of the dumbbell are likely to correspond to two domains. We examined the role of the two sets of ATPase motifs by analyzing the in vitro activity of ATPgammaS binding, AMPPNP-induced hexamerization, thermostability, and phosphorylation of KaiC and by in vivo rhythm assays both in wild type KaiC (KaiCWT) and KaiCs carrying mutations in either Walker motif A or deduced catalytic Glu residues. We demonstrated that 1) the KaiC subunit had two types of ATP-binding sites, a high affinity site in N-terminal ATPase motifs and a low affinity site in C-terminal ATPase motifs, 2) the N-terminal motifs were responsible for hexamerization, and 3) the C-terminal motifs were responsible for both stabilization and phosphorylation of the KaiC hexamer. We proposed the following reaction mechanism. ATP preferentially binds to the N-terminal high affinity site, inducing the hexamerization of KaiC. Additional ATP then binds to the C-terminal low affinity site, stabilizing and phosphorylating the hexamer. We discussed the ...Continue Reading

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Citations

Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Connie PhongMichael J Rust
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael J Rust
May 15, 2008·Genes & Development·Yohko KitayamaTakao Kondo
Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hee-Jin KangMasaru Tanokura
Apr 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeroen S van ZonPieter Rein ten Wolde
Mar 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Taeko Nishiwaki-OhkawaTakao Kondo
Oct 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Peng-Juan LiangYu-Long Shen
Mar 28, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Reiko MurakamiMasahiro Ishiura
Nov 27, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Shunsuke KurosawaMasahiro Ishiura
Apr 20, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J Valencia SMasahiro Ishiura
Aug 12, 2006·Biochemical and Biophysical Research Communications·Fumio HayashiMasahiro Ishiura
Mar 29, 2014·Current Opinion in Microbiology·Gopal Pattanayak, Michael J Rust
Mar 16, 2017·PLoS Computational Biology·Joris PaijmansPieter Rein Ten Wolde
May 26, 2009·Journal of Biological Rhythms·Mirre J P Simons
Feb 15, 2018·The Journal of Biological Chemistry·Jeffrey A SwanCarrie L Partch
Jun 21, 2019·Journal of Biological Rhythms·Young M JeongYong-Ick Kim
Aug 2, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Reiko MurakamiMasahiro Ishiura
Sep 22, 2019·International Journal of Molecular Sciences·Reiko MurakamiKoichi Kato

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