Rolling adhesion of human NK cells to porcine endothelial cells mainly relies on CD49d-CD106 interactions

Transplantation
Mårten K J SchneiderJörg D Seebach

Abstract

Acute vascular rejection in pig-to-primate xenotransplantation involves recognition and damage of porcine (po) endothelial cells (EC) by human (hu) leukocytes, probably including natural killer (NK) cells. To study such interactions we analyzed rolling and static adhesion of hu NK cells to po EC. The effects of blocking hu and po adhesion molecules on the adhesion hu NK cells to po EC monolayers was analyzed under shear stress (10 min, 37 degrees C, 0.7 dynes/cm2) or under static conditions (10 min, 37 degrees C). All used cell populations were phenotypically characterized by flow cytometry. Blocking of CD106 on po EC or its ligand CD49d on hu NK cells decreased rolling adhesion of both fresh and activated hu NK cells by more than 75%. Masking of CD62L on fresh but not activated hu NK resulted in a 44% decrease in rolling adhesion, in line with the diminished cell surface expression of CD62L upon activation. Antibodies to CD31, CD54, CD62E, and CD62P on EC or CD11a, CD18, and CD162 on NK cells had only minor effects on rolling adhesion. The adhesion of the FcgammaRIII- hu NK cell line NK92 to po EC was inhibited by 95% after masking po CD106 whereas antibodies to po CD31, CD54, CD62E, or CD62P had no effect, thereby excluding e...Continue Reading

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Citations

Jan 6, 2010·Acta histochemica·Nuray AcarRamazan Demir
Nov 11, 2008·Transplant Immunology·Shengqiao LiAn D Billiau
Apr 15, 2008·American Journal of Reproductive Immunology : AJRI·Angela SantoniAngela Gismondi
Oct 27, 2010·Annals of the New York Academy of Sciences·Iurii KobozievMatthew B Grisham
Feb 8, 2018·Journal of Immunology Research·Gisella Puga YungJörg D Seebach
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marit SaethreTom E Mollnes
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bettina C BaumannJörg D Seebach
Jan 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberta SommaggioCristina Costa

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