Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses

Journal of Virological Methods
Daniela HaibleHolger Jeske

Abstract

Better, easier and cheaper than polymerase chain reaction (PCR) or antibody detection, rolling circle amplification (RCA) using the bacteriophage varphi 29 DNA polymerase allows for a reliable diagnosis of geminiviruses and presumably all viruses with small single-stranded circular DNA genomes. The results show the efficiency of this technique in characterizing viral DNA components of several geminiviruses from experimental and natural host plant sources. The advantages are: (a) that no expensive devices are necessary, (b) simple handling, (c) detection of all infecting circular DNA components without any knowledge of sequence information in a single step, and (d) low costs per reaction. In addition, RCA-amplified viral DNA can be characterized by restriction fragment length polymorphism analysis and directly sequenced up to 900 bases in a single run circumventing cloning and plasmid purification. This shortcut will considerably accelerate genomics of at least gemini-, nano- and circoviruses in the future.

References

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Mar 29, 2003·Trends in Plant Science·Shahid MansoorJohn Stanley
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Jan 5, 2005·Virology·Barbara AlberterHolger Jeske

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Citations

Jun 21, 2008·Archives of Virology·N NahidR W Briddon
Dec 21, 2010·Archives of Virology·Patrícia Soares WyantHolger Jeske
Nov 15, 2011·Archives of Virology·Stephen J WylieMichael G K Jones
Feb 7, 2012·Archives of Virology·Prem A RajagopalanRadhamani Anandalakshmi
Mar 3, 2012·Archives of Virology·Belén Márquez-MartínEnrique Moriones
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·P JyothsnaV G Malathi
Dec 3, 2010·The ISME Journal·Kimberly P TuckerMya Breitbart
May 10, 2013·Phytopathology·Maher Al RwahnihMysore R Sudarshana
Sep 20, 2011·Applied and Environmental Microbiology·Kyoung-Ho Kim, Jin-Woo Bae
Aug 19, 2008·Applied and Environmental Microbiology·Kyoung-Ho KimJin-Woo Bae
Jun 27, 2012·Journal of Virology·Björn KrenzKeith L Perry
Aug 7, 2009·Journal of Virology·Ioana GrigorasBruno Gronenborn
Aug 13, 2011·Journal of Virology·T PaprotkaH Jeske
Dec 19, 2013·PLoS Genetics·Carolina Megumi MizunoRohit Ghai
Feb 18, 2014·Virus Research·Judith Bach, Holger Jeske

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