Rolling circle replication of hepatitis delta virus RNA is carried out by two different cellular RNA polymerases

Journal of Virology
Thomas B MacnaughtonMichael M C Lai

Abstract

Hepatitis delta virus (HDV) contains a viroid-like circular RNA that is presumed to replicate via a rolling circle replication mechanism mediated by cellular RNA polymerases. However, the exact mechanism of rolling circle replication for HDV RNA and viroids is not clear. Using our recently described cDNA-free transfection system (L. E. Modahl and M. M. Lai, J. Virol. 72:5449-5456, 1998), we have succeeded in detecting HDV RNA replication by metabolic labeling with [32P]orthophosphate in vivo and obtained direct evidence that HDV RNA replication generates high-molecular-weight multimeric species of HDV RNA, which are processed into monomeric and dimeric forms. Thus, these multimeric RNAs are the true intermediates of HDV RNA replication. We also found that HDV RNA synthesis is highly temperature sensitive, occurring most efficiently at 37 to 40 degrees C and becoming virtually undetectable at temperatures below 30 degrees C. Moreover, genomic HDV RNA synthesis was found to occur at a rate roughly 30-fold higher than that of antigenomic RNA synthesis. Finally, in lysolecithin-permeabilized cells, the synthesis of full-length antigenomic HDV RNA was completely resistant to high concentrations (100 microg/ml) of alpha-amanitin. In ...Continue Reading

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Citations

Jun 25, 2003·Biochemical and Biophysical Research Communications·Feng-Ming LinMei Chao
Nov 8, 2002·Journal of Molecular Biology·Amy Diegelman-Parente, Philip C Bevilacqua
Apr 23, 2003·Trends in Microbiology·John M Taylor
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Jun 17, 2008·Nature Structural & Molecular Biology·Dirk HausseckerMark A Kay
Nov 6, 2009·Journal of Virology·Chung-Hsin TsengMichael M C Lai
Nov 23, 2007·Journal of Virology·Jinhong ChangJohn Taylor
Sep 13, 2003·Journal of Virology·Cromwell T Cornillez-Ty, David W Lazinski
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Apr 17, 2016·Journal of Hepatology·Camille Sureau, Francesco Negro
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Dec 1, 2009·Viruses·Chung-Hsin Tseng, Michael M C Lai
Jan 1, 2010·Viruses·Valerie Greco-Stewart, Martin Pelchat

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