PMID: 6168611Jan 1, 1981Paper

Romanowsky dyes and romanowsky-Giemsa effect. 1. Azure B, purity and content of dye samples, association (author's transl)

Histochemistry
E ZipfelH W Zimmermann

Abstract

Azure B is the most important Romanowsky dye. In combination with eosin Y it produces the well known Romanowsky-Giemsa staining pattern on the cell. Usually commercial azure B is strongly contaminated. We prepared a sample of azure B-BF4 which was analytically pure and had no coloured impurities. The substance was used to redetermine the molar extinction coefficient epsilon (v)M of monomeric azur B in alcoholic solution. In the maximum of the long wavelength absorption at v = 15.61 kK (lambda = 641 nm) the absorptivity is epsilon (15.61)M = (9.40 +/- 0.15) x 10(4)M-1 cm-1. This extinction coefficient may be used for standardization of dye samples. In aqeuous solution azur B forms dimers and even higher polymers with increasing concentration. The dissociation constant of the dimers, K = 2,2 x 10(-4)M (293 K), and the absorption spectra of pure monomers and dimers in water have been calculated from the concentration dependence of the spectra using an iterative procedure. The molar extinction coefficient of the monomers at 15.47 kK (646 nm) is epsilon (15.47)M = 7.4 x 10(4)M-1 cm-1. The dimers have two long wavelength absorption bands at 14.60 and 16.80 kK (685 and 595 nm) with very different intensities 2 x 10(4) and 13.5 x 10(4)...Continue Reading

References

Aug 1, 1975·Journal of Clinical Pathology·P N MarshallS M Lewis
Jan 1, 1978·The Histochemical Journal·P N Marshall
Nov 1, 1975·Journal of Clinical Pathology·P N MarshallS M Lewis
Jan 1, 1979·Clinical and Laboratory Haematology·D Wittekind
Jul 1, 1974·Stain Technology·P N Marshall, S M Lewis
Jul 1, 1960·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K B TAYLOR

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Citations

Jan 1, 1994·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·E K Schulte
Feb 4, 2009·Virchows Archiv : an International Journal of Pathology·Arne WarthJürgen Burhenne
Jan 1, 1987·Histochemistry·R Müller-Walz, H W Zimmermann
Jan 1, 1987·Histochemistry·C WinzekH Baumgärtel
Jan 1, 1984·Histochemistry·P JakobsenS Treppendahl
Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D WittekindG Frank

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